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云南省EV71的分离鉴定和生物学特性分析

Identification and characterization of enterovirus 71 isolated in Yunnan province
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摘要 目的对云南省采集的8份EV71标本进行分离鉴定，进行生物学特征分析。方法采集HFMD患儿的标本，然后使用RT-PCR法进行鉴定，对鉴定为阳性的标本进行病毒分离，分离得到的病毒株在Vero细胞中培养，并进行VP1基因测序。用病毒免疫小鼠，免疫后血清进行中和试验和交叉保护试验。结果8份标本均为EV71，并均在Vero细胞中都表现出细胞病变；部分免疫血清效价可高达1：10000以上。结论8株病毒在Vero细胞中有良好的适应性；免疫血清对A和C4基因型保护效果明显。 Objective Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province. Methods Collecting specimens from cases of Hand, Foot and Mouth Disease （HFMD） , samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera. Result All samples were identified as EV71, and eight strains had cytopathie effect （ CPE ） in vero cells, some antisera titers were more than 1 ： 10 000. Conclusion Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.
作者 赵世文 尹卫东 寸建萍 高强 尹洁 刘国军 陆林 徐闻
机构地区 云南省疾病预防控制中心 北京科兴生物制品有限公司 昆明医科大学
出处 《中华实验和临床病毒学杂志》 CAS CSCD 2013年第4期263-265,共3页 Chinese Journal of Experimental and Clinical Virology
关键词 肠道病毒属 基因型 交叉感染 Enterovirus Genotype Cross-infedtion
分类号 R4 [医药卫生—临床医学]
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参考文献12

  • 1Huang WP, Lin TL, Kuo CY, et al. The circulation of subgenogroups B5 and C5 of cnterovirus 71 in Taiwan from 2006 to 2007. Virus Res, 2008,137:206-212.
  • 2Solomon T, Lewthwaite P, Perera D, et al. Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet Infect Dis, 2010,10:778-90.
  • 3贾卉,蔡芳,高强,张建三,井申荣.EV71抗原ELISA定量检测方法的建立[J].中国生物制品学杂志,2010,23(1):87-90. 被引量：18
  • 4MeMinn P, Lindsay K, Pemra D, et al. Phylogenetio analysis of entemvirus 71 strains isolated during linked epidemics in Malaysia, Singapore and Western Australia. J Virol, 2001,75: 7732-7738.
  • 5Cardosa M J, Perera D, Brown B A, et al. Molecular epidemiology of human enterovims 71 strains and recent outbreaks in the Asia-Pacific region:comparative analysis of the VPI and VP4 genes. Emerg Infect Dis. 2003,9:461-468.
  • 6Chen K T, Chang H L, Wang ST, et al. Epidemiologic features of hand-foot-mouth disease and herpangina caused by enterovirus 71 in Taiwan, 1998-2005. Pediatrics, 2007,120 : e244-252.
  • 7Grandien M Fosgren M, Ehmet A. Enterovirua. In: LennetteEH, Lennette DA, Lennette ET, eds. Diagnostic procedures for vi-ral, rickettsial and chlamydial infections. 7th ed. Washing-ton, DC: American Public Health Association, 1995. 279-298.
  • 8Yin-Murphy M, Tan KL, Lim GN, et al. Polivorirus neutralizing antibody in infants and cord blood. Ann Acad Med Singapore, 1993,22:281-285.
  • 9郝翊,秦川.人肠道病毒71型亚单位疫苗的研制[J].中国比较医学杂志,2010,20(6):7-12. 被引量：6
  • 10刘国军,吴燕,田炳均,寸建萍,尹洁,姜黎黎,马跃,陆林,徐闻.云南省2009～2010年肠道病毒71型的基因特征分析[J].病毒学报,2012,28(4):389-393. 被引量：8

二级参考文献32

  • 1Yeo WM, Chow VT. The VP1 structural protein of enterovirus 71 interacts with human ornithine decarboxylase and gene trap ankyrin repeat. Microb Pathog, 2007, 42 (4): 129-137.
  • 2Chen HL, Huang JY, Chu TW, et al. Expression of VPI protein in the milk of transgenic mice: a potential oral vaccine protects against enterovirus 71 infection. Vaccine, 2008, 26 (23): 2882- 2889.
  • 3Luo ST, Chiang PS, Chao AS, et al. Enterovirns 71 maternal antibodies in infants, Taiwan. Emerg Infect Dis, 2009, 15 (4):581- 584.
  • 4Iwai M, Masaki A, Hasegawa S, et al. Genetic changes of coxsackievirus A16 and enterovirus 71 isolated from hand, foot, and mouth disease patients in Toyama, Japan between 1981 and 2007. Jpn J Infect Dis, 2009, 62 (4): 254-259.
  • 5Zhu SB, Weng KZ, Zhou HW, et al. Severe enterovirus 71-associated hang-foot-and-mouth disease complicated by brainstem encephalitis: report of 2 cases. Chin J Contemporary Pediatrics, 2009, 11 (6): 502-503.
  • 6Li X, Mao C, Ma S, et al. Generation of neutralizing monoclonal antibodies against Enterovirus 71 using synthetic peptides. Biochem Biophys Res Commun, 2009, 390 (4): 1126-1128.
  • 7Wang SM, Liu CC. Enterovirus 71: epidemiology, pathogenesis and management. Expert Rev Anti Infect Ther, 2009, 7 (6): 735-742.
  • 8Ang LW, Koh BK, Chan KP, et al. Epidemiology and control of hand, foot and mouth disease in Singapore, 2001-2007. Ann Acad Med Singapore, 2009, 38 !2): 106-112.
  • 9Wu TC, Wang YF, Lee YP, et al. Immunity to avirulent enterovirus 71 and coxsackie A16 virus protects against enterovirus 71 infection in mice. J Virol, 2007,81(19):10310-10315.
  • 10Tung WS, Bakar SA, Sekawi Z, et al.DNA vaccine constructs against enterovirus 71 elicit immune response in mice. Genet Vaccines Ther, 2007, 5 (6): 1-13.

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中华实验和临床病毒学杂志
2013年 第4期

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