摘要
目的:观察NF-κB特异性抑制剂PDTC对人肺腺癌A549细胞增殖、细胞周期、凋亡及星形细胞上调基因-1(AEG-1)表达的影响。方法:用不同浓度(25、50、100、200μmol/L)的PDTC分别处理A549细胞,对照组不给PDTC。应用MTT法检测处理24、48、72h后细胞增殖抑制率,流式细胞术检测处理24h后细胞周期的变化,Ho-echst33342荧光染色检测处理24h后细胞的凋亡情况,RT-PCR和Westernblot检测处理24h后细胞AEG-1mRNA和蛋白的表达。结果:经PDTC处理的A549细胞增殖明显受抑,呈时间和浓度依赖性(F时间=531.981,F浓度=388.475,P<0.05);与对照组相比,PDTC处理24h后A549细胞G0/G1期细胞比例上升(P<0.05),细胞形态出现明显凋亡改变;随着PDTC浓度的增加,癌细胞内AEG-1mRNA和蛋白的表达水平降低(F=275.162、59.473,P<0.05)。结论:PDTC可抑制A549细胞的增殖,诱导其凋亡,其机制可能与PDTC抑制NF-κB、AEG-1的表达有关。
Aim： To explore the effect of pyrrolidine dithiocarbamate（PDTC） on proliferation,cell cycle,apoptosis of human lung cancer cell line A549 and expression of astrocyte elevated gene-1（AEG-1） .Methods： A549 cells were treated by PDTC with different concentrations（25,50,100,200 μmol/L） for different time（24,48,72 h） respectively.MTT assay was used to detect the inhibition rates of cell growth.Flow cytometry was used to analyze the changes of cell cycle.Hoechst33342 staining was used to observe the apoptosis of cells with the fluorescence microscope.RT-PCR and Western blot were used to detect the expression of AEG-1.Results： PDTC inhibited the A549 cell proliferation in a timeand dose-dependent manner（Ftime=531.981,Fconcentration=388.475,P0.05） .In contrast to the control,the percentage of cells in G 0/G1 phase increased significantly in PDTC group（P0.05） with apparent apoptotic morphological changes.The mRNA and protein expressions of AEG-1 were reduced with increasing concentration of PDTC （F=275.162 and 59.473,P0.05） .Conclusion： PDTC can inhibit the proliferation,and induce the apoptosis of A549 cells.The mechanism may be related to the down-regulated expressions of NF-κB and AEG-1 gene induced by PDTC.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2013年第4期485-488,共4页
Journal of Zhengzhou University(Medical Sciences)
