摘要
作者通过辣根过氧化物酶标记抗体（HRP-IgG）蛋白分子固定在金纳米粒子（GNP）表面的方法,制备得到一种高活性的酶-金纳米复合物（HRP-IgG-GNP）。将该HRP-IgG-GNP应用于酶联免疫分析（ELISA）方法中,针对去甲基睾酮（NT）,建立了一种高灵敏的纳米增强ELISA方法。该方法的检测灵敏度达到0.01 ng/mL,线性范围为0.03~10 ng/mL。与传统ELISA方法相比,该方法的灵敏度提高了10倍。该纳米增强ELISA方法有望开发高灵敏免疫分析方法中获得广泛应用。
Conjugating enzyme with nanoparticles is an effective approach to enhance the characteristics of enzyme molecule in biosensor. A highly active enzyme-nanoparticle complex,HRP-IgG GNP,was prepared by immobilizing horseradish peroxidase labeled antibody （HRP-IgG） onto the surface of gold nanoparticle （GNP）. The HRP-IgG GNP complex was applied to the enzyme-linked absorbent immunoassay （ELISA） and a highly sensitive nanopaticle-enhanced ELISA method was developed against nortestosterone （NT）. The sensitivity of the novel method reached 0.01 ng/mL and the linear range was 0.03-10 ng/mL, the nanopaticle-enhanced ELISA method was 10 times more sensitive than the traditional ELISA method. The above method has promising applications for developing highly sensitive immunoassay methods.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2013年第6期656-660,共5页
Journal of Food Science and Biotechnology
基金
教育部博士点新教师基金项目(20110093120003)
江南大学大学生实践创新项目
