摘要
为明确电子束辐照对采后草莓灰霉病菌(Botrytis cinerea)菌体致病能力的影响,采用活体接种法测定了经不同剂量电子束辐照处理的B.cinerea菌体对采后草莓果实的致病力,采用DNS法测定了电子束辐照对B.cinerea菌体果胶酶及纤维素酶的产生及活性的影响。结果表明,电子束辐照可以显著降低B.cinerea菌体的致病力,接种草莓果实的发病率及病情指数明显降低,3.0kGy处理的该菌菌体接种草莓果实后,第5天时的发病率和病情指数仅为15.00%和11.39,显著低于对照的91.67%和77.78;2.0kGy以上电子束处理可明显抑制B.cinerea菌体果胶酶及纤维素酶等致病相关酶活性,第3天和第5天时,3.0kGy处理的该菌PMG活性分别比对照降低37.65%和56.46%、Cx和BG的活性分别较对照降低60%以上;活体草莓接种酶液后,2.0kGy处理的菌体产生的酶液处理的草莓硬度明显高于对照草莓,保持较好的抗病能力。电子束辐照可以降低B.cinerea菌体致病力,明显抑制其致病相关酶的活性,有效控制草莓采后灰霉病的发生。
In order to define the effect of electron beam irradiation on pathogenicity of Botrytis cinerea mycelia,the disease parameters of strawberry fruits inoculated with mycelia of B.cinerea irradiated by electron beam with different dose were tested in vivo,the production and activity of pectinase and cellulase secreted by B.cinerea irradiated by electron beam were also tested by DNS method.The results showed that pathogenicity of irradiated B.cinerea decreased significantly.The disease incidence and disease index of strawberry inoculated by irradiated B.cinerea after 3 days and 5 days was only 15.00% and 11.39 compared to control（91.67% and 77.78）,respectively.The activity of pectinase and cellulase of B.cinerea irradiated by electron beam with above 2.0kGy were inhibited obviously.The activity of PMG of irradiated B.cinerea decreased by 37.65% and 57.46% compared with control 3 days and 5 days cultured at 20℃,and the activity of CX and BG decreased by over 60% than that of control.The hardness of strawberry treated by enzyme solution which producted by B.cinerea irradiated by electron beam at 2.0kGy was higher than that of control strawberry and it mainted disease resistance well.Electron beam can inhibited the pathogenic enzyme activity and pathogenicity of B.cinerea obviously.So it can control gray mold of postharvest strawberry effectively.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第8期1156-1161,共6页
Journal of Nuclear Agricultural Sciences
基金
上海市科技兴农重点攻关项目[沪农科攻字(2007)第9-1号]
