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针对肺癌标志物CYFRA21-1检测的三通道高精度光学检测系统 被引量:2

A three-channel high-precision optical detecting system for lung cancer marker CYFRA21-1
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摘要 构建了一种针对肺癌标志物细胞角蛋白19的可溶性片段CYFRA21-1的三通道高精度光学检测系统。系统基于磁酶免疫分析实验的特点,通过巧妙的机械结构和精确的控制系统设计,利用FPGA高频计数功能、NIOS核控制功能,配合自己编写的上位机软件,实现了对CYFRA21-1的定量检测;在确保高精度的前提下,实现了3个通道的同时检测,大大节省了检测时间,提高了检测效率。通过多次实验验证,系统3个通道间干扰小、一致性好,相对偏差小于1%。在CYFRA21-1抗原溶液检测实验中,系统在CYFRA21-1浓度为2-100ng/mL范围内线性关系良好,相关系数为0.979。系统具有小型便携、灵敏度高、检测时间短和重复性好等特点,为疾病标志物的快速检测提供了强有力的工具。 A three-channel high-precision optical detecting system for lang cancer marker CYFRA21-1 is described. This system is based on the magnetic enzyme-linked immunoassay, depends on the skillful mechanical design and precise controlling system, uses FPGA to count the high-frequency pulse and NIOS to control the whole system,and achieves the quantitative detection of CYFRA21-1 with the help of my own PC software. On the premise of high precision,three channels can be used in the same time,which can increase the efficiency of detection significantly. After experimental verification, the interference among the three channels is little and the consistency is good, and the relative deviation is lower than 1 %. The CYFRA21 1 sample with concentration of 2-100 ng/mL was measured and the correlation coefficient between CYFRA21-1 concentrations is 0. 979. This system is small, has high sensitivity and short detecting time, so it could be a powerful tool for the rapid disease marker detection.
出处 《光电子.激光》 EI CAS CSCD 北大核心 2013年第9期1849-1854,共6页 Journal of Optoelectronics·Laser
基金 国家自然科学基金(61027001 61002037 61101048) 国家重点基础研究发展计划(2001CB933202) 中国科学院战略性先导专项(XDA06020101)资助项目
关键词 肺癌 CYFRA21—1 三通道 光子计数 lung cancer CYFRA21-1 three- channel photon- counting
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  • 1张格,张亚秋,付家庆.生物超弱发光的光子成像及其检测方法研究[J].农机化研究,2006,28(11):81-83. 被引量:4
  • 2刘亚峰,原永平.D—二聚体与肺癌临床分期之间关系的研究[J].中国现代医药杂志,2007,9(2):80-81. 被引量:8
  • 3魏继锋,张凯.光子成像计数技术及其新进展[J].激光与光电子学进展,2007,44(7):27-32. 被引量:5
  • 4查人俊,何长清,曾羝闻,等.现代肿瘤诊断与治疗[M].北京:人民军医出版社,1999:60-63.
  • 5王志新,左媛,陈维中,张楚毅.肺癌患者血浆D-二聚体异常改变的双重意义[J].中国慢性病预防与控制,1997,5(2):73-74. 被引量:13
  • 6Satoh H, Ishikawa H, Kurishima K, et al. Cut-off levels of NSE to diferentiate SCLC from NSCLC [ J 1. 0ncol Rep, 2002, 9(3) :581-583.
  • 7Poola I, Graziano SL. Expression of neuron-specific enolase, chromogranin A, synaptophysin and Leu-7 in lung cancer cell lines [ J ]. J Exp Clin Cancer Res, 1998,17(2) :165-173.
  • 8Bonner JA, Sloan JA, Rowland KM, et al. Significance of neuron-specific enolase levels before and during therapy for small cell lung cancer [ J ]. Clin Cancer Res, 2000,6 ( 2 ) : 597-601.
  • 9Flokman J, Watson K, Ingber D, et al. Induction of angiogenesis during the transition from hyperplasia to neoplasia [ J ]. Nature, 1989,339 ( 6219 ) :58-61.
  • 10Matei D, Kelich S, Cao L, et al. PDGF BB induces VEGF secretion in ovarian cancer [ J ]. Cancer Biol Ther,2007,6 (12) : 1951-1959.

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  • 1韩强,张华勇.BSP、CEA和Cyfra21-1联合检测在NSCLC骨转移中的诊断价值[J].青海医药杂志,2012,42(5):2-5. 被引量:1
  • 2李文兵,骆海军,王鹏,刘跃.ECT联合CYFRA21-1、NTx、BSP检测在诊断肺癌骨转移中的价值[J].湖北医药学院学报,2013,32(2):111-114. 被引量:1
  • 3王雅杰,张锟,方芳,姚见儿,齐军,吕虹,李国祥,郑艳颖,周雪雷,蔡勇华,李久彤,孙艳艳,索凤霜,康熙雄.应用液体芯片技术联合检测癌胚抗原、19片段角蛋白和神经元特异性烯醇化酶对于肺癌诊断价值的研究[J].中国实验诊断学,2006,10(6):588-592. 被引量:6
  • 4Steven HLin,Ritsuko Komaki, LIAO Zhong-xing,et al.Proton beam therapy and concurrent chemotherapy for e-sophageal cancer [J]. Internationa 丨Journalof RadiationOncology. Biology. Physics,2012,83(3) :345-351 .
  • 5GUO PULI Ke. Trends in esophageal cancer mortality inChina during 1987-2009 : Age, period and birth cohort an-alyzes[J]. Cancer Epidemiology,2012, (36):99-105.
  • 6FENG Shang-yuan,CHEN Rong,LIN Ju-qiang,et al. Gas-tric cancer detection based on blood plasma surface-en-hanced Raman spectroscopy excited by polarized laserlight[J]#NAME?Bioelectronics, 2011,(26):3167-3174.
  • 7TU Qiang,CHANG Chang. Diagnostic applications of Ra-man spectroscopy [J]. Nanomedicine: Nanotechnology,Biology,and Medicine,2012,(8) -545-558.
  • 8Marc Vendrell,Kaustabh Kumar Maiti,Kevin DhaliwaUetal. Surface-enhanced Raman scattering in cancer detec-tion and imaging [J]. Trends in Biotechnology,2013, 31(4):249-257.
  • 9Nabiev I R, Efremov R GChumanov G D. Surface-en-hanced Raman scattering and its application to study ofbiological molecules [J]. Soviet Physics Uspekhi,1988,(31):241-247.
  • 10Chumanov G D, Efremov R G, Nabiev I R. Surface- en-hanced Raman spectroscopy of biomolecules[J]. Journalof RamanSpectroscopy ,1990,(21): 43-48.

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