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正交试验优选土茯苓总黄酮的最佳提取工艺 被引量:1

The Optimum Technique for Extracting Total Flavonoids from Smilax glabra Roxb. by Orthogonal Test
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摘要 [目的]优选土茯苓总黄酮的最佳提取工艺。[方法]在单因素试验的基础上,以土茯苓总黄酮的收率为指标,采用正交试验法优选土茯苓总黄酮的最佳提取工艺条件。[结果]土茯苓总黄酮的最佳提取工艺为:料液比为1∶20,乙醇浓度为60%,提取温度为95℃,回流提取2次,每次2 h;在此条件下,总黄酮的收率为8.34%。[结论]该工艺操作简单、稳定、可行。 Abstract [ Objective ] To optimize the extracting technology of total flavonoids from Smilax glabra Roxb.. [ Method ] On the basis of single factor test, using the yield of total flavonoids as indicator, the optimum extracting technology of total flavonoids was obtained by orthogonal test. [ Result ] The optimum extracting technology was as follows:solid-liquid ratio 1:20, 60% ethanol, extracting 2 times at 95 ~C for 2 hours. The yield of total flavonoids was 8.34% under the above conditions. [ Conclusion] The extracting technology is simple, stable and feasible.
出处 《安徽农业科学》 CAS 2013年第17期7468-7470,共3页 Journal of Anhui Agricultural Sciences
关键词 土茯苓(RHIZOMA SMILACIS GLABRAE) 总黄酮 提取工艺 正交试验 Smilax glabra Roxb. Total flavonoids Extraction technolo^w: Orthozonal test
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