摘要
目的研究道地产区磐安延胡索Corydalis Rhizoma的化学成分。方法采用硅胶、Sephadex LH-20等柱色谱方法进行分离纯化,通过化合物的理化性质和谱学数据鉴定其结构。结果从延胡索95%乙醇提取物中分离得到20个生物碱,分别鉴定为左旋四氢黄连碱(1)、四氢非洲防己胺(2)、延胡索乙素(3)、紫堇球碱(4)、异紫堇球碱(5)、8-三氯甲基-7,8-二氢黄连碱(6)、延胡索甲素(紫堇碱,7)、8-酮基黄连碱(8)、左旋紫堇根碱(9)、去氢延胡索甲素(10)、13-甲基巴马亭红碱(11)、氧化海罂粟碱(12)、原阿片碱(13)、降氧化北美黄连次碱(14)、四氢小檗碱(15)、二去氢海罂粟碱(16)、黄海罂粟灵碱(17)、黄连碱(18)、巴马亭(19)、小檗碱(20)。结论化合物6、9和16均为首次从延胡索中分离得到。首次应用2D NMR技术对化合物6和16的13C-NMR、1H-NMR信号进行了全归属。
Objective To study the chemical constituents from Corydalis Rhizoma in Pan'an county as characteristically regional traditional Chinese medicinal materials. Methods The constituents were separated and purified by chromatography on silica gel and Sephadex LH-20 columns, as well as the chemical structures of alkaloids were determined by physicochemical properties and spectral data analyses. Results Twenty alkaloids were obtained and identified as tetrahydrocoptisine (1), tetrahydrocolumbamine (2), tetrahydropalmatine (3), corybulbine (4), isocorybulbine (5), 8-trichloromethyl-7, 8-dihydrocoptisine (6), corydaline (7), 8-oxocoptisine (8), (?)-corypalmine (9), dehydrocorydaline (10), 13-methylpalmatrubine (11), oxoglaucine (12), protopine (13), noroxyhydrastinine (14), tetrahydroberberine (15), didehydroglaucine (16), pontevedrine (17), coptisine (18), palmatine (19), and berberine (20). Conclusion Compounds 6, 9, and 16 are isolated from Corydalis Rhizoma for the first time. All 13C-NMR and 1H-NMR data of compounds 6 and 16 are assigned for the first time using 2D NMR.
出处
《中草药》
CAS
CSCD
北大核心
2013年第16期2200-2207,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重点资助项目(30830118)
“十二五”国家科技支撑专项(2011BAI03B01,2011BAI07B08)