摘要
背景:异种生物骨经适当理化方法处理及高温锻烧后能够形成具有与人骨结构相近的天然网状孔隙结构的陶瓷样异种骨,具有三维立体孔隙-网架结构,有利于种子细胞的黏附、增殖。目的:观察以高温煅烧骨及骨髓间充质干细胞复合构建形成的组织工程化骨修复牙槽骨缺损的可行性。方法:以羊骨髓间充质干细胞为种子细胞,高温煅烧骨为支架材料,复合构建形成组织工程化骨。全麻下分批次拔除羊双侧下颌第一前磨牙,去除远中根与第二前磨牙近中根间牙槽嵴间隔,形成5mm×5mm×5mm骨缺损区域。将12只实验羊随机等分为组织工程化骨组和单纯煅烧骨组,分别在左侧下颌术区放组织工程化骨和单纯煅烧骨,所有动物右侧均作为空白对照组。结果与结论:高温煅烧骨呈白垩色,保留了天然松质骨的多孔状空间。孔隙率(66.10±1.32)%,孔径范围137.44-538.72μm。干细胞接种到煅烧骨24h后可见细胞黏附支架上,7d后分泌胞外基质,细胞与基质分界不清。X射线可见组织工程化骨组和单纯煅烧骨组植入材料包埋在术区,煅烧骨边缘有一圈低密度阴影。苏木精-伊红染色实验侧骨小梁形成,对照侧无明显骨生成。提示应用骨髓间充质干细胞复合煅烧骨构建的组织工程化骨可较好的修复牙槽骨缺损,是修复小范围骨缺损理想的种子细胞和支架材料。
BACKGROUND： Following physicochemical treatment and high-temperature calcinations, heterogeneous biological bone becomes a ceramic-like heterologous bone forming a similar structure to the human bone that is a natural network pore structure, which is conducive to seed cell adhesion and proliferation. OBJECTIVE： To observe the feasibility of constructing tissue-engineered bone through combination of sintered bone and bone marrow mesenchymal stem cells to repair alveolar defects. METHODS： Sheep bone marrow mesenchymal stem cells as seed cells were combined with the high temperature sintered bone as scaffold materials to construct tissue-engineered bone. Under general anesthesia, sheep bilateral mandibular first premolars were removed in batches, the alveolar ridge space between the distal root and mesial root of the second premolar to form a bone defect area of 5 mm×5 mm×5 mm. Twelve experimental sheep were equally randomized into tissue-engineered bone group and sintered bone group, which were implanted with tissue-engineered bone and sintered bone, respectively, at the left surgical area of the mandible. The right surgical area was considered as blank control group. RESULTS AND CONCLUSION： After high-temperature calcinations, the sintered bone was chalk in color, exhibiting a porous structure as the natural cancellous bone. The porosity was （66.10±1.32）%, and the pore size was between 137.44 μm and 538.72 μm. After 24 hours of bone marrow mesenchymal stem cells inoculated to the sintered bone, a large number of cells are visible adherent to the scaffold; up to day 7, extracellular matrix was secreted and there was no clear boundary between the cells and the matrix. X-ray films showed that the tissue-engineered bone and pure sintered bone implants were embedded in the surgical area, and there was a low-density shadow at the edge of the sintered bone. Hematoxylin-eosin staining showed bone trabecular formation at the experimental side, but no obvious bone formation at the controlled side. Tissue-engineered boneprepared by bone marrow mesenchymal stem cells and sintered bone can better repair sheep alveolar bone defects, which is an ideal seed cell and scaffold material for small range bone defects.
出处
《中国组织工程研究》
CAS
CSCD
2013年第34期6089-6096,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(81060088)
课题名称:三维打印构建组织工程化牙槽骨的实验研究
新疆维吾尔自治区科技厅自然科学基金(2011211A073)
课题名称:绿色荧光蛋白标记的组织工程骨修复颌骨缺损及其成骨能力的观测研究
新疆医科大学第一附属医院青年基金项目(2012QN21)
课题名称:注射型PRF凝胶复合材料对兔拔牙窝剩余牙槽嵴保存的实验研究~~
关键词
生物材料
组织工程口腔材料
组织工程化骨
牙槽骨
骨髓基质干细胞
煅烧骨
骨缺损
国家自然科学基金
biomateriais
tissue-engineered oral materials
tissue-engineered bone
alveolar bone
bone marrowstromal stem cells
sintered bone
bone defect
National Natural Science Foundation of China
