摘要
背景:中药来源的三七总皂甙苷可通过增加缺血部位血管再生及骨折愈合,因此推测载三七总皂苷的中药支架具有促进骨缺损部位骨痂再生的作用,但其生物相容性尚不清楚。目的:制备三七总皂苷-羟基磷灰石/壳聚糖骨修复支架,评估其生物相容性。方法:采用原位复合和冷冻干燥技术制备载三七总皂苷量分别为0,0.1,1,10mg的羟基磷灰石/壳聚糖复合材料,采用扫描电镜观察复合支架形貌,并测试其机械性能。①细胞增殖实验:以4种载药羟基磷灰石/壳聚糖复合材料浸提液培养第3代兔骨髓间充质干细胞,以正常培养的细胞为对照,MTT法检测各组细胞A值。②溶血实验:在抗凝兔血中分别加入4种载药羟基磷灰石/壳聚糖复合材料浸提液,酶标仪检测各组A值。③热源实验:经兔耳缘静脉分别注射4种载药羟基磷灰石/壳聚糖复合材料浸提液与生理盐水,检测兔体温升高值。结果与结论:载药羟基磷灰石/壳聚糖复合材料含有大量直径为110μm的三维多孔结构,三七总皂苷载药过程对复合材料的孔隙率、孔径、密度无显著影响,但却降低了其断裂强度和弹性模量,载药量越大效果越明显。单纯的羟基磷灰石/壳聚糖复合材料细胞相容性良好,载药后的复合材料明显抑制了细胞增殖,载药量越大效果越明显。载药前后的复合材料均具有良好的血液相容性。载药前后的复合材料均具有一定的热源作用,但符合热源实验的检测合格标准。
BACKGROUND： Panax notoginseng saponins promotes bone repair by improving vascular proliferation. Therefore, the scaffolds carrying panax notoginseng saponins were supposed to be used to improve bone repair at the bone defect region. However, the biocompatibility of scaffolds remains unclear. OBJECTIVE： To evaluate the biocompatibility of panax notoginseng saponins-hydroxyapatite/chitosan scaffolds. METHODS： A new bone repair scaffold has been generated by thoroughly mixtures of 0, 0.1, 1, 10 mg panax notoginseng saponins and chitosan/hydroxyapatite using in-situ composite technique and freeze-drying technique. Morphology and mechanical property of the scaffold were observed under a scanning electron microscope. （1） Cell proliferation test： rabbit bone marrow mesenchymal stem cells of passage 3 were cultured in four kinds of drug loaded hydroxyapatite/chitosan composite material leaching liquor. Cells normally cultured were considered as controls. 3-（4, 5-Dimethylthiazol-2-yl） 2, 5-diphenyl tetrazolium bromide was used to measure absorbance value of cells in each group. （2） Hemolysis test： Rabbit anticoagulated blood was added with four kinds of drug loaded hydroxyapatite/chitosan composite material leaching liquor. Absorbance values weremeasured using a microplate reader in each group. （3） Pyrogen test： The four kinds of drug loaded hydroxyapatite/chitosan composite material leaching liquor and saline were respectively injected into ear vein of rabbits, and the increase of rabbit body temperature was detected. RESULTS AND CONCLUSION： Drug loaded hydroxyapatite/chitosan composite material contained three dimensional porous structure of 110 pm in diameter. Drug loading process of panax notoginseng saponins did not significantly affect the porosity, pore size and density of the composite material, but decreased its breaking strength and elastic modulus. The larger amount of drug loading showed a more obvious effect. Simple hydroxyapatite/chitosan composite material had good cellular compatibility. The composite material after drug loading obviously suppressed cell proliferation, and the larger amount of drug loading showed a more obvious effect. The composite material had good blood compatibility before and after drug loading. The composite material had good pyrogen effects before and after drug loading, but accorded with acceptable quality level of pyrogen test,
出处
《中国组织工程研究》
CAS
CSCD
2013年第34期6116-6122,共7页
Chinese Journal of Tissue Engineering Research
基金
广东省中医药局立项课题(2010339-B)~~
关键词
生物材料
材料生物相容性
三七总皂苷
羟基磷灰石
壳聚糖
支架
生物相容性
骨组织工程
省级基金
biomaterials
material biocompatibility
panax notoginseng saponins
hydroxyapatite
chitosan
scaffold
biocompatibility
bone tissue engineering
provincial grants-supported paper
