摘要
目的探讨去细胞组织工程猪肺动脉带瓣管道的免疫学特性。方法新鲜猪肺动脉带瓣管道组织3份,分别为对照组、去细胞组与明胶嵌合组,对照组不作处理,去细胞组、明胶嵌合组采用胰蛋白酶+Triton X-100对新鲜猪肺动脉带瓣管道进行去细胞处理,明胶嵌合组在去细胞处理后行明胶嵌合再处理;对3组瓣膜及管壁组织行大鼠皮下包埋实验,8周后观察材料的组织相容性和生长情况,Van Kosaa银染色法定性分析、原子吸收光度计法定量分析组织的钙化程度。结果与对照组比较,去细胞组炎性细胞浸润明显减少,明胶嵌合组仅有轻微组织反应;去细胞组及明胶嵌合组材料组织相容性好,8周后有纤维细胞长入;定性分析结果显示,去细胞组与明胶嵌合组瓣叶和管壁组织中呈黑色颗粒的钙盐沉积较对照组明显下降,明胶嵌合组钙盐沉积较去细胞组明显下降;定量分析结果显示,去细胞组肺动脉管壁组织及瓣膜组织钙盐含量((1.39±0.11)、(0.60±0.07)mg/g)较对照组((19.47±0.83)、(16.68±0.74)mg/g)明显下降,较明胶嵌合组((0.14±0.03)、(0.06±0.01)mg/g)明显升高(P<0.05)。结论采用明胶嵌合去细胞方法构建的组织工程猪肺动脉带瓣管道,其免疫原性明显下降,有一定生长潜能,有望成为一种新的、理想的组织工程肺动脉带瓣管道支架材料。
Objective To explore the immune properties of decellularized porcine tissue engineering pulmonary valved conduits. Methods The fresh porcine pulmonary valved conduits were divided into three groups： control group, decellularized group and gelatin mosaieing group. Decellularized group and gelatin mosaicing group were decellularized with trypsin plus Triton X-100, gelatin mosaicing group was retreated by gelatin mosaicing, and controal group received no treatment. The valves and wall tissue of these three groups were implanted subcutaneously in mice and extirpated at 8th week to study the biocompatibility and biodegradibility. Van Kosaa silver staining qualitative analysis and atomic absorption spectrometer quantitive analysis were adopted to determine the extent of calcification of the tissue. Results Compared with control group, the infiltration of inflammatory cells significantly decreased in decellularized group, and the valves and wall tissue in gelatin mosaieing group showed a very slight tissue reaction. The latter two groups had good histocompatibility and fibroblast grew by the end of the 8th week. Qualitative analysis showed that the black particles of calcium deposition in the valves and wall tissue decreased significantly in deceliularized group and gelatin mosaicing group compared with control group, and decreased more significantly in deeellularized group than that in gelatin mosaicing group. The quantitative analysis showed that the contents of calcium in the valves and wall tissue were （1.39±0. 11） mg/g and （0.60 ± 0.07） mg/g in deceilularized group, obviously lower than those in control group （（ 19.47±0.83） mg/g and （16. 68±0.74） mg/g）, and higher than those in gelatin mosaicing group （（0.14±0.03） mg/g and （0. 06±0.01） mg/g） （P〈0.05）. Conclusion The new gelatin mosaic acellular porcine pulmonary valved conduits, with lower heterogeneic pulmonie immunogenicity and good growth potential, would become a new and ideal tissue engineering pulmonary valved conduits scaffold. Key Words ： Pulmonary valved conduits~ porcine; tissue engineering; deceliularization
出处
《中华实用诊断与治疗杂志》
2013年第9期881-883,共3页
Journal of Chinese Practical Diagnosis and Therapy
关键词
免疫学
肺动脉带瓣管道
猪
组织工程
去细胞
Pulmonary valved conduits
porcine
tissue engineering
decellularization
