摘要
目的探讨围生期暴露双酚A(bispheno1 A,BPA)对青春期雌性子代大鼠体重、糖代谢及细胞炎症因子的影响。方法将21只清洁级SD妊娠大鼠分为3组,分别为对照(含1%无水乙醇的水)组和低(1μg/ml)、高(10μg/ml)浓度BPA暴露组,每组7只。从妊娠第6天至哺乳期(出生后20 d),母鼠采用自由饮水方式进行染毒;断乳后,雌性仔鼠采用自由饮水(不含BPA)方式继续喂养30 d。测定雌性仔鼠体重、空腹血糖、胰岛素水平及血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素6(interleukin-6,IL-6)水平,并计算胰岛素抵抗指数。结果各浓度BPA暴露组雌性仔鼠体重、空腹血糖、血清胰岛素水平和胰岛素抵抗指数、TNF-α、IL-6水平均明显高于对照组,差异有统计学意义(P<0.05,P<0.01)。结论围生期暴露BPA可引起子代雌性大鼠体重增高和胰岛素抵抗,并可诱发机体炎症反应。
Objective To explore the effects of perinatal exposure to bisphenol A（BPA） on body weight and inflammatory factors in adolescent female offspring rats. Methods A total of 21 pregnant Sprague-Dawley rats were randomly divided into three groups, seven rats in each group, the control group （1% of anhydrous alcohol with water）,low dose （1 μg/ml） and high dose （10 μg/ml） groups of BPA exposed through drinking water,from gestation day 6 to the end of lactation respectively. Female offspring drank water without BPA for 30 days after weaning. Body weight,fasting serum glucose,insulin,necrosis factor-α （TNF-α） and interleukin-6 （IL-6） levels were determined, and insulin resistance index was calculated in female offspring after postnatal day 50. Results There were significantly higher body weight,serum glucose,insulin,and insulin resistance index,TNF-α and IL-6 levels in BPA-exposed female rats compared with the controls （P〈0.05 or P〈0.01）. Conclusion Perinatal exposure to BPA may result in body weight gain and insulin resistance,and induce inflammatory response in female offspring.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2013年第8期665-667,共3页
Journal of Environment and Health
基金
国家自然科学基金(81072311)
