摘要
采用冷水、温水、稀碱对臭参的地下和地上部分多糖分别进行提取,然后用苯酚-硫酸法测定其多糖的含量。测得用冷水、温水、稀碱提取的臭参地下部分的多糖分别为15.81%、14.64%、4.51%,地上部分的多糖分别为4.18%、1.96%、0.27%。同时还研究了臭参多糖对DPPH自由基、·OH、O2-·的清除作用及其总还原能力。实验结果表明,臭参多糖的还原能力及清除自由基的能力随着浓度的增加而增大。浓度为11.196mg/mL时,对DPPH自由基的清除率可达97.8%。因此,臭参多糖具有明显的抗氧化活性。
The polysaccharides were extracted from the above-and below-ground parts of Codonopsis bulleyana Forrest ex Diels by cold and hot water, and dilute alkaline solution, respectively. The content of polysaccharide was measured by Phenol-sulfuric acid method, that is 4.18%, 1.96%, and 0.27% of the above-ground, 15.81%, 14.64%, and 4.51% of the below-ground, which was extracted by cold, warm water, and dilute alkaline solution, respectively. Further, we studied the reducing capacity and scavenging activity of the polysaccharides from Codonopsis bulleyana Forrest ex Diels （CBFP） for the free radical of DPPH, .OH and 02-. The results display that the reducing capacity and scavenging activity of polysaccharides from CBFP increased with the concentration increasing. The clear ratio can even reach 98.7% for DPPH when concentrated to 11.196 mg/mL. Thus confirmed the polysaccharide form CBFP possesses excellent anti-oxidant activity.
出处
《食品科技》
CAS
北大核心
2013年第8期282-286,共5页
Food Science and Technology
基金
云南民族大学民族药资源化学国家民委-教育部重点实验室合作项目(MZY1103)
曲靖市地方特色食品研究所课题
关键词
臭参多糖
提取
含量测定
抗氧化能力
polysaccharide from Codonopsis bulleyana Forrest ex Diels
extract
content measurement
anti-oxidant activity
