摘要
建立了干性样品中多菌灵残留量的检测方法。采用含1%乙酸的乙腈提取样品,提取液经弗罗里硅土吸附净化后,采用高效液相色谱-紫外可见光检测器串联荧光检测器进行检测。采用ZORBAX SB-C18柱,水-甲醇-乙腈为流动相,等梯度洗,以紫外可见光检测定性,荧光检测外标法定量。多菌灵在质量分数0.02～2.00mg/kg范围内线性良好(r2=0.9998),检测限(S/N=3)为0.01mg/L,定量限(S/N=10)为0.030mg/kg。在0.02～0.10mg/kg质量分数水平加标范围内,回收率范围为90.0%～106.0%,相对标准偏差小于10.0%。
A method was developed for the determination of carbendazim residue in dried-vegetable by high performance liquid chromatography tandem double detectors coupled with dispersive extraction. The sample was extracted with 1% acetic acid acetonitrile, cleaned up by dispersive solid phase extraction using florisil as an adsorbent,and analyzed with high performance liquid chromatography equipped with UV and FLD detectors in which quantitative and qualitative analysis were carried out by FLD detector in external standard method and UV detector, respectively. The carbendazin residue was separated by a ZORBAX SB-C18 column, using water-methanol-acetonitrile as mobile phase in isocratic elution. The results indi quan 0.02 tificati cared that the calib on（LOQ） of 0. 030 0.10 mg/kg with RSDs ration curve mg/kg. The less than 10. was linear in the range of 0.02-2.00 mg/kg with the limit of spiked recoveries were from 90.0%-106.0% in the range of 0%.
出处
《分析科学学报》
CAS
CSCD
北大核心
2013年第4期565-568,共4页
Journal of Analytical Science
基金
福建出入境检验检验局科技项目(FK2011-61)
关键词
多菌灵
残留量
干性蔬菜
高效液相色谱
串联双检测器
Carbendazim
Residue
Dried samples
High per
ormance liquid chromatography
Tandem double dec-tectors
