摘要
根据已发表的 5 2 / 70株传染性腔上囊病病毒 (IBDV)基因组序列 ,设计并合成了一对特异扩增IBDVVP2基因的引物。以超强毒IBDV (vvIBDV )致弱株GZ2 0基因组为模板利用RT PCR技术扩增出了 1.5kb的cDNA产物 ,将VP2基因克隆于 pUC119质粒上 ,得到重组 pUC119质粒。并对VP2基因全序列测定 ,序列分析和聚类分析表明 ,该致弱株与无毒疫苗株PBG98和弱毒株CU 1非常相似 ,而与经典强毒株、超强毒株和变异株相差较大。这提示该致弱株属于标准血清Ⅰ型弱毒株。
According to the published sequence of IBDV strain 52/70, a pair of primers that can amplify the cDNA of protective antigen VP2 gene was designed and synthesized . By RT PCR , a single DNA fragment of about 1.5 kb was obtained from attenuated strain GZ20 of vvIBDV. Then the VP2 cDNA was cloned into pUC119 at SmaI site. The nucleotide sequence of the expected VP2 gene was determined by Sanger's DNA sequencing method, and then the amino acid sequence was deduced. Both the nucleotide sequence and amino acid sequence were compared with six published sequence of VP2 gene of IBDV serotype Ⅰ strain . It was shown that GZ20 strain was mostly closely related to the low pathogenic strain CU 1 and non pathogenic strain PBG98 but different from the very viralent strain UK661 、 OKYM and other serotype I strains .These suggested that GZ20 belongs to standard I strain and has low pathogenicity.
出处
《中国兽医科技》
CSCD
2000年第6期3-7,共5页
Chinese Journal of Veterinary Science and Technology
