摘要
采用巢式-PCR、DNA克隆测序技术,对西双版纳地区野生与栽培绞股蓝根内丛枝菌根真菌(AMF)进行群落结构多样性研究.试验共获得551个含有丛枝菌根真菌18S rDNA片段的克隆子,经限制性片段长度多态性分析,得到100个RFLP类型,将其划分为20个序列类型,分属于7个科.将20个序列类型的相关序列与GeneBank数据库进行比对,有5个可以鉴定到种,分别为Glomus viscosum、Claroideoglomus etunicatum、Racocetra tropicana、Acaulospora spinosa、Acaulospora mellea.与MaarjAM数据库中序列进行比对,12个可鉴定为虚拟分类分子种,其中7个未在孢子形态学鉴定方法中获得.西双版纳地区野生与栽培绞股蓝根内丛枝菌根真菌群落组成差异极显著.野生绞股蓝根内Glo-2、Amb-1、Para-1为优势类群,而栽培绞股蓝根内Glo-3、Glo-8、Glo-10、Div-1为优势类群.Claroideoglomeraceae和Ambisporaceae仅在野生样本中出现,而Diversisporaceae仅在栽培样品中出现.
By using nested-PCR, DNA cloning, and sequencing techniques, this paper studied the diversity of the community structure of arbuscular mycorrhizal fungi （AMF） in wild and cultured Gynostemma pentaphyllum roots. A total of 551 clones containing 18S rDNA genes of AMF were ob- tained from the roots. After the analysis of the restriction fragment length polymorphism, 100 differ- ent RFLP types were obtained, which were further divided into 20 AMF phylotypes belonging to seven families. The comparison of the sequences of 20 AMF phylotypes with the GenBank database showed that there were 5 AMF phylotypes having high similarity to the sequences of reported AMF species Glomus viscosum , Claroideoglomus etunicatum , Racocetra tropicana , Acaulospora spinosa , and Acaulospora mellea, respectively. These sequences were then assessed for the similarities against the MaarjAM database, and 12 phylotypes showed high similarity to the corresponding mo- lecular virtual taxa, of which, 7 phylotypes were not obtained by the morphological identification of soil asexual spores. Statistical analysis indicated that there were significant differences in the AMF community between wild and cultured G. pentaphyllum roots. The analysis of relative abundance data indicated that Glo-2, Amb-1, and Para-1 were the dominant phylotypes in wild G. pentaphyllum roots, while Glo-3, Glo-8, Glo-10, and Div-1 were the prevalent phylotypes in cultured ones. Claroideoglomeraceae and Ambisporaceae were only detected in wild G. pentaphyl- lum roots, and Diversisporaceae was only identified in cultured ones.
出处
《应用生态学报》
CAS
CSCD
北大核心
2013年第9期2503-2510,共8页
Chinese Journal of Applied Ecology
