摘要
目的:评价白介素(interleukin,IL)-17A在结直肠癌及腺瘤间质中的表达及意义.方法:回顾性收集我院消化内镜室2009-2011年行肠镜检查后诊为结直肠癌与结直肠腺瘤患者的临床病理资料及组织标本,通过免疫组织化学方法评价IL-17A的表达及其意义,同时评价其与腺瘤组织-连接素(catenin)表达状态的相关性.结果:结直肠癌53例,IL-17A阳性细胞数为21.6±14.4,高表达率54.2%;结直肠腺瘤48例,IL-17A阳性细胞数为20.42±17.16,高表达率45.8%;溃疡性结肠炎14例,IL-17A阳性细胞数为20.24±12.17,高表达率50.0%;正常对照12例,IL-17A阳性细胞数为6.85±4.66,高表达率0.0%,癌、腺瘤与溃疡性结肠炎中的表达均显著高于正常对照(P<0.01),但三者之间没有统计学差异(P>0.05).结直肠癌中,IL-17A的表达与结肠癌的部位、分化程度、Duke's分期、淋巴结转移、微血管密度(microvessel d e n s i t y,M V D)以及K i-67表达均不相关(P>0.05).结直肠腺瘤中,IL-17A的表达与腺瘤组织学类型、MVD、部位、大小、颜色、形态、是否多发及患者是否伴发结直肠癌均不相关(P>0.05);重度异型增生的腺瘤组织IL-17A的表达明显高于轻度和中度异型增生的腺瘤组织(29.62±26.77与16.98±10.62),但差异没有统计学意义(P>0.05).-catenin胞核表达的结直肠腺瘤组织中IL-17A计数(29.11±6.16)明显高于胞浆(16.95±7.99)及胞膜(15.10±11.46)表达的结直肠腺瘤(P值分别为0.038与0.021),结直肠腺瘤中IL-17A的表达强度与-catenin的表达部位显著相关(P=0.002).结论:结直肠腺瘤与结直肠癌间质中存在IL-17A高表达,IL-17A可能具有促进结直肠腺瘤组织中-catenin胞核转位的作用.
AIM： To investigate the expression and role of interleukin-17 （IL-17） in colorectal cancer and adenoma.
METHODS： Tissue samples taken from patients with colorectal cancer or adenoma who were treated in our gastrointestinal endoscopy unit between 2009 and 2011 were used in this retrospective study. The expression of IL-17A in the above tissue samples was detected by immunohistochemistry. The relationship between IL-17A expression and clinicopathological characteristics and β-catenin localization was analyzed.
RESULTS： The positive rate of IL-17A expression was significantly higher in colorectal cancer, colorectal adenoma and ulcerative colitis than in normal colorectal tissue （54.2%, 45.8%, 50.0% vs 0.0%, all P 〈 0.01）. IL-17A expression was not associated with tumor location, degree of differentiation, Duke’s stage, lymph node metastasis, MVD or Ki-67 expression in colorectal cancer （all P 〉 0.05）. IL-17A expression was also not associated with histological type, MVD, concomitant colorectal cancer, location, size, color, number or shape of adenomas （all P 〉 0.05） in colorectal adenoma. The expression of IL-17A was higher in adenomas with high-grade dysplasia than in those with mild to moderate dysplasia, but the difference was not significant （29.62 ± 26.77 vs 16.98 ± 10.62, P 〉 0.05）. The expression of IL-17A was significantly higher in adenomas with nuclear expression of β-catenin than in those with cytoplasmic or membrane expression of β-catenin （29.11 ± 6.16 vs 16.95 ± 7.99, 15.10 ± 11.46, P = 0.038, 0.021）.
CONCLUSION： IL-17A expression is elevated in colorectal cancer and adenomas and may mediate nuclear translocation of β-catenin.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第25期2522-2529,共8页
World Chinese Journal of Digestology
基金
首都医科大学附属北京世纪坛医院青年科学基金资助项目
No.2011-q01~~
