摘要
以天祝县青海云杉天然种群种子为研究对象,通过对种子生活力测定,分析种子萌发对温度、光照、水分和盐分胁迫的响应,为引种提供参考数据。结果表明:①种子的生活力为72%。②在黑暗条件下,温度小于10℃种子不萌发,最适萌发温度25/15℃(72%);种子在24 h黑暗和24 h光照条件下的萌发率显著高于14 h光照/10 h黑暗。③当NaCl胁迫浓度为50 mmol.L-1时,可促进种子萌发,当胁迫浓度大于50 mmol.L-1时,抑制种子萌发;胁迫解除后,随着胁迫浓度的增加,恢复萌发率(RGR)先增加后减小,胁迫浓度为400 mmol.L-1时,RGR达到最大值13%。④随着PEG-6000试剂胁迫渗透势的增加,种子萌发显著减少,在-0.9 MPa时,种子不能萌发;胁迫解除后,RGR先增加后减少,在-0.9 MPa时,达到最大值35%。
Seeds were collected from the natural population of Picea crassifolia Kom.in Tianzhu County,Wuwei City,Gansu Province,China in November 2010.Seed vigor was tested by TTC,the experiments were conducted to investigate the response of seed germination to temperature,light,moisture stress and salinity stress,and some references were finally provided for transplanting P.crassifolia Kom.from Tianzhu to Minqin County.The results are as follows： ① The vigor of P.crassifolia Kom.seeds was 72%;② Under a complete dark,seeds could not germinate when temperature was lower than 10 ℃,and the optimal temperature for germination was 25/15 ℃（72%）;germination rate of P.crassifolia Kom.seeds under the 24-hour complete dark and 24-hour light were significantly higher than that under 14-hour light and 10-hour dark;③ Seed germination could be improved when NaCl concentration was 50 mmol·L-1,but it could be restricted when NaCl concentration was higher than 50 mmol·L-1;seed germination could be recovered after salinity stress was removed,the recovery germination rate（RGR） was increased at first and then decreased with the increase of NaCl concentration,and RGR reached its maximum（13%） when NaCl concentration was 400 mmol·L-1;④ Seed germination was significantly decreased with the increase of PEG-6000 osmotic potential,and seeds could not germinate when the osmotic potential was-0.9 MPa;after PEG-6000 stress was removed,RGR was increased at first and then decreased with the increase of PEG-6000 concentration,and it reached its maximum（35%） when the osmotic potential was-0.9 MPa.
出处
《干旱区研究》
CSCD
北大核心
2013年第5期877-881,共5页
Arid Zone Research
基金
国家自然科学基金项目(30960062)
甘肃省技术研究与开发专项计划项目(0709TCYH035)
国家林业局公益性科研院所专项资金项目(CAFYBB200700X)
