摘要
目的用HPLC-ELSD法测定益心调脉合剂中黄芪甲苷的含量。方法采用Lichrospher C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈—水(32∶68)为流动相,漂移管温度为60℃,流速1.0 mL·min-1。结果黄芪甲苷在0.050 3～1.006 g·L-1范围内呈良好的线性关系,标准曲线为lgA=1.609 4 lgC+6.218 0(r=0.999 6);加样回收率为99.1%,RSD%为1.2%。结论该法简便准确,灵敏度高,重复性好,可用于益心调脉合剂的质量控制。
Objective To determine the content of astragaloside IV in Yixintiaomai mixture by HPLC-ELSD. Methods A Lichrospher C 18
（4.6 mm×250 mm,5 μm） was used as the analytical column and mobile phase was acetonitrile-water （ 32：68 ） at a flow rate of 1.0 mL·min^-1. The temperature of the drift tube was 60℃. Results The linearity equation of astragaloside IV was lgA = 1. 609 4 lgC ＋ 6. 218 0 （ r = 0.999 6）. The average recovery was 97.9%. RSD was 1.2% ,and the linear range was 0. 0503 - 1. 006g·L^-1. Concul-sions The method is simple, rapid and reproducible, which can be used for the quality control of Yixintiaomai mixture.
出处
《安徽医药》
CAS
2013年第9期1500-1501,共2页
Anhui Medical and Pharmaceutical Journal
