摘要
以单细胞的真核模式生物裂殖酵母 (S .pombe)为材料 ,研究钙与细胞增殖的关系。结果表明 ,外源钙浓度的增加能明显缩短群体生长的延滞期 ,促进细胞增殖。添加Ca2 + 螯合剂EGTA ,在培养基pH≥ 5 4条件下能完全抑制S .pombe细胞的增殖 ,终止细胞周期的正常运转 ;而CaCl2 对EGTA抑制S .pombe细胞增殖具有恢复作用。这表明EGTA终止S .pombe细胞增殖是由于缺Ca2 + 而引起的。此外 ,EGTA与A2 31 87配合使用可以显著降低S .pombe细胞内Ca2 + 含量 ,可见维持S .pombe胞内钙量的稳定 。
Taking an example of unicellular eukaryotic organisms fission yeast Schizosaccharomyces pombe(S.pombe) ,the effects of Ca 2+ on cell proliferation were studied.The results indicated that the cell proliferation of S.pombe stimulated apparently with increasing of Ca 2+ level.The cell proliferation of S.pombe could be completely inhibited by EGTA at pH≥5 4.The addition of CaCl 2 was found to alleviate the inhibition of cell proliferation by EGTA.The combined use of EGTA and A23187 apparently decreased the content of total Ca 2+ of S.pombe ,and completely inhibited the cell proliferation both in lag and log phase,showing that stabilization of Ca 2+ content in the cells of S.pombe was important for the cell proliferation.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2000年第2期53-56,共4页
Journal of Nanjing Agricultural University
