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肺癌Th1/Th2免疫反应状态的研究 被引量:7

Study of reaction state of Th1/Th2 of patients with lung cancer
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摘要 为探讨肺癌细胞及肺癌患者外周血单个核细胞 (PBMC) Th1/ Th2的免疫反应状态 ,为肿瘤的免疫治疗提供依据 ,以 IL - 2和 IFN- r代表 Th1型细胞因子 ,IL - 4、IL - 6和 IL - 10代表 Th2型细胞因子 ,用 RT- PCR方法检测了三种人肺癌 (鳞癌、腺癌、小细胞癌 )细胞株及 2 3例肺癌患者 PBMC中 Th1/ Th2型细胞因子 m- RNA的表达。结果显示 ,三种肺癌细胞的 Th1型因子 IFN- r和 IL- 2均无表达 ,而 Th2型因子 IL- 4、IL- 6、IL- 10均表达 ;肺癌患者PBMC中 IL- 4、IL- 6和 IL- 10表达阳性率均较正常对照组显著增高 ,P均 <0 .0 5。而 IL- 2无 1例表达 ,IFN- r仅 1例表达。证实肺癌细胞株及肺癌患者 PBMC均呈现 The reaction state of Th1/Th2 in human lung cancer cells and peripheral blood mononuclear cells(PBMC)from lung cancer patients was studied for immunological treatment of cancer the m RNA expression of type Th1 cytokines(IL 2,IFN r)and type Th2 cytokines(IL 4,IL 6,IL 10)was detected by reverse transcriptase PCR in the lung cancer cell strains(including sq uamate and adenogenous and small cell lung cancer)and the PBMC from 23 lung cancer patients and 19 healthy controls The result showed that none of the human lung cancer cell strains expresses m RNA of IL 2 and IFN r,whereas all type Th2 cytokines(IL 4,IL 6,IL 10)were presented The m RNA expressing rate of IL 4,IL 6,IL 10 of PBMC from lung cancer patients was obviously higher than that from healthy control,P<0 05 respectively No m Rna of IL 2 was expressed in patients's PBMC,and a positive expression of IFN r was found in only one patient's PBMC It is suggests that there is a predominant reaction of type Th2 in both lung cancer cells and PBMC from lung cancer patients
出处 《山东医药》 CAS 北大核心 2000年第19期4-5,共2页 Shandong Medical Journal
基金 山东省科委立项课题!(9812116501)
关键词 肺癌 细胞因子 基因检测 T辅助细胞亚群 Lung cancer Cytokine Gene detection Th1/Th2
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同被引文献33

  • 1李岩,梁婧,刘文波,许晓群,赵跃然.化疗对胃癌Th1和Th2类细胞因子漂移的影响及临床意义[J].中华肿瘤杂志,2004,26(12):732-734. 被引量:20
  • 2张少云,王春利,张素英,马炎炎,郑启英,郭石平,杨凯峰,范兴平.流式细胞微球芯片捕获技术在食管癌患者Th1/Th2细胞因子谱型分析中的意义[J].中华检验医学杂志,2005,28(5):502-503. 被引量:8
  • 3梁婧,李岩,刘晓琳,许晓群,赵跃然,董恒磊(校对).化疗对结肠癌Th1和Th2类细胞因子基因表达的影响及意义[J].中国肿瘤临床,2006,33(16):916-919. 被引量:7
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