摘要
蔗果低聚糖(FOS)作为一种益生元物质,由于其具有极好的生物和功能性质,受到人们极大的关注。具有独立知识产权的菌株Aspergillus niger CGMCC No.6640被发现能利用蔗糖制备FOS。为利用A.niger 6640的全细胞制备FOS,以蔗糖为底物,利用高效液相法对该菌株的全细胞生物转化参数进行了研究。利用色谱柱RezexRCM-Monosaccharife Cat的高效液相法能同时检测催化产物中蔗果四糖(或蔗果五糖)、蔗果三糖、蔗糖、葡萄糖和果糖的浓度,其保留时间分别为8.403 min,8.853min,9.705 min,11.473min和14.683 min。全细胞生物催化剂浓度和底物浓度对FOS产量有正面的影响。而CaCl2浓度对FOS含量有负面影响。当全细胞生物催化剂浓度,初始反应pH、反应温度、反应时间、蔗糖浓度分别为60 g/L、7.0、33℃、40 h和600 g/L的最佳条件下,FOS含量为314.60 g/L。总之,Aspergillus niger CGMCC No.6640全细胞生物催化剂能有效地制备FOS,为其工业化提供依据。
Fructooligosaccharides （FOS） have received particular interest because of their excellent biological and functional properties for using as a prebiotic compound. The strain Aspergillus niger CGMCC No. 6640 with independent intellectual property rights was found capable of producing FOS using sucrose as the substrate. To produce FOS using free-whole-ceU biotransformation by A. niger 6640, the effects of production parameters on the biotransformation of sucrose were investigated by HPLC with the column of Rezex RCM-Monosaccharife Cat. The catalysate concentration of the Nystose （or 1F-Fructofuranosylnystose）, kestose, sucrose, glucose and fructose were simultaneously detected, and their retention time was 8.403 min, 8.853 min, 9.705 min, 11.473 min and 14.683 min, respectively. The free-whole-cell concentration and substrate concentration positively affected the maximum FOS yield. However CaCI2 concentration negatively affected the FOS yield. The free-whole-cell concentration, the initial reaction pH, the temperature for catalysis, biotransformation lime and sucrose concentration were 60 g/L, 7.0, 33 ℃, 40 h and 600 g/L, respectively. Under the optimal conditions were as follows, the FOS contents reached 314.60 g/L. In sunurmry, the free-whole-cell biocatalyst of A. niger 6640 can effectively produce FOS indicating a potential for industrial production.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第8期1967-1972,1966,共7页
Modern Food Science and Technology
基金
国家自然科学基金资助项目(31101254)
广东省自然科学基金资助项目(No.S2011010005679)
关键词
蔗果低聚糖
黑曲霉
β-呋喃果糖基转移酶
全细胞生物催化剂
生物转化
fructooligosaccharides
aspergillus niger
β-fructosyltransferase
the biocatalyst of free-whole-cell
biotransformation
