摘要
节杆菌YB6(Arthrobacter spp.)为番茄青枯病拮抗菌株,其发酵滤液稳定性测定结果表明,该发酵滤液对热、酸碱及蛋白酶K敏感,在60℃及其以上温度失去抑菌活性;强酸强碱条件和蛋白酶K浓度1.5 mg/mL以上均会使其活性显著下降;对光照和紫外线稳定,5 h紫外线照射和35 000 lx光照条件下抑菌活性无显著变化。抗菌谱研究显示,对测试的8种病原菌均有抑制效果,其中对甘蔗黑粉病和小麦赤霉病抑制效果较强,抑菌率分别达88.6%和80.2%。以蘸根对番茄青枯病的防效最好,其防治率达到65.0%。
A rthrobacter YB6 strain is an antagonistic bacterium against Ralstonia solanacearum. In the stability test, the ferment filtrat was sensitive to heat, acid-alkali and protease K, it had no antibacterial activity when the temperature was above 60~C, the antibacterial activity would decrease significantly under the condition of strong acid-alkali or the concentration of protease K over 1.5 mg/mL. However, the ferment filtrat had strong stability against light and UV. The antibacterial actiwity was still stable under the condition of UV irradiation for 5 h or 35 000 L light. The ferment fihrat had wide antibacterial spectrum, and had inhibiting effect against 8 pathogen in the test. It had the best inhibiting effect against Ustilago scitaminea Syd. and Fusarium graminecrum, the bacteriostatic rate achieved 88.6% and 80.2%, respectively. The inhibition activity against R. solanacearum by the dipping inoeulum method was the best in pot experiments and its biocontrol rate reached 65.0%.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第18期75-78,共4页
Guangdong Agricultural Sciences
基金
广东省科技成果转化启动项目(2010B060600007)
广东省农科院土壤肥料研究所科研创新平台建设(2012B060600010)
关键词
节杆菌
番茄青枯病
发酵滤液
抑菌活性
防治效果
Arthrobacter
Ralstonia solanacearum
ferment fihrate
antibacterial activity
control effect
