摘要
青蟹双顺反子病毒-1（mudcrabdicistrovirus-1，MCDV-1）和青蟹呼肠孤病毒（mudcrabreovirus，MCRV）是对养殖拟穴青蟹有较强致病性的2种病原，可导致高死亡率。文章采用双重巢式PCR检测法对2012年阳江地区5月-10月捕捞的野生拟穴青蟹（Scyllaparamamosain）病毒感染情况进行调查。结果显示，阳江地区2012年5月～10月的野生青蟹群体一直保持较高的病毒携带率（44．83％-100％），其中MCDV-1检出率最高的月份为6月、8月和9月，MCRV检出率最高的月份为5月-6月和8月～9月；以单独感染类型为主的月份为5月、7月和10月（〉44．83％），以双阳性感染类型为主的月份为6月、8月和9月（〉82．76％）。由此可见，2种病毒的流行月份为5月～6月和8月～9月；在流行月份期间2种病毒常常协同感染野生拟穴青蟹。
Mud crab dicistrovirus-1 （MCDV-1） and mud crab reovirus （MCRV） are of serious pathogenicity to the mud crab （Scylla paramamosain）, which cause high mortality and great economic losses in the mud crab culture. A survey was carried out to determine the virus infection in the wild mud crab captured along Yangjiang coast from May to October in 2012. The viruses were detected by a duplex nested-PCR. The results showed that high infection rate of virus were observed in the wild mud crab （44. 83% - 100% ） from May to October in 2012. The high rate of MCDV-1 infection appeared in June, August and September. The high rate of MCRV infec- tion appeared in May, June, August and September. In May, July and October, single infection dominated （ 〉44. 83% ） in wild mud crabs; while in June, August and September, coinfection （ 〉82. 76% ） dominated. These results reveal that the peak epidemic months of two viruses are May-June and August-September. During these months, MCDV-1 and MCRV often jointly infect wild mud crab.
出处
《南方水产科学》
CAS
CSCD
北大核心
2013年第5期137-141,共5页
South China Fisheries Science
基金
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2012TS06)
广东省科技计划项目(2011A020102002)
广东省教育部产学研结合项目(2010B090400519)
