摘要
为明确河南省烟草赤星病病原菌的种类,从河南省不同烟区采集烟草赤星病典型病斑,分离病原菌并且做致病性测定,对确定为病原菌的菌种按国际标准方法进一步做形态和分子鉴定。结果显示分离到的85株菌株中60株被证明是烟草赤星病病原菌,从形态学上鉴定为三个种,分别是链格孢Alternaria alternata、长柄链格孢Alternaria longipes和鸭梨链格孢Alternaria yaliinficiens。其中鸭梨链格孢Alternaria yaliinficiens引起烟草赤星病属首次报道。三个种的代表菌株的5.8S rDNA ITS基因序列间及已报道的链格孢Alternaria alternata,长柄链格孢Alternaria longipes和鸭梨链格孢Alternaria yaliinficiens的5.8S rDNA ITS区均没有较大差异,证明该基因在链格孢小孢子种间较保守,不适合区分这些链格孢种。
Typical disease spot specimens were collected from different areas and pathogen isolation and pathogenicity tests were carried out to determine tobacco brown spot pathogen species in Henan province. Results showed that 60 out of 85 strains isolated from disease spot were confirmed to be pathogens of tobacco brown spot by Koch＇s postulation. They were identified as three species of Altemaria by morphology characteristics using standard methods, i.e. Alter naria alternata, Alternaria longipes and Alternaria yaliinficiens. This is the first report ofA. yaliinficiens causing tobacco brown spot. The 5.8S rDNA-ITS gene sequences of representative strains of the three species and other strains of Alternaria alternata, Alternaria longipes and Alternaria yaliinficiens show no clear difference, which proved that the gene is not suitable for distinguishing these small-spore Alternaria species.
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2013年第4期73-77,共5页
Acta Tabacaria Sinica
基金
中国烟草总公司项目(110200902065)
河南省烟草有害生物调查研究
郑州市科技创新领军人才项目(121PLJRC530)
