摘要
对裙带菜7个单克隆进行TRAPD分析,从20个引物中筛选出16个能产生稳定的扩增片段的引物, 共产生64条扩增片段,其中56条呈现多态,多态位点比例高达87.5%.根据这些引物扩增的指纹图谱,计 算了各材料之间的遗传距离(D),并由此进行了聚类分析.结果显示,各品系之间的遗传距离在0.4419 ~ 0.742 0之间。裙带菜种内遗传变异丰富,其遗传距离与地理分布没有必然的联系.
RAPD is employed to analyze genetic variations of seven gametophyte clones of Unda ria Pinnatifida. Twenty primers have been used and sixteen of them can give 64 reproducible amplified fragments, of which 56 are polymorphic. Dendrograms constructed from RAPD data showed that there is a high genetic variation inUndaria Pinnatifida. (D=0.4419~0.7420) and the genetic distance has no direct relation to the geographic origin.
出处
《海洋通报》
CAS
CSCD
北大核心
2000年第6期24-28,共5页
Marine Science Bulletin