摘要
在基础饲料中添加0、0.8、1.2、1.6、2.0、2.4 g/kg(饲料)的香菇多糖,连续饲喂体质量为(40.0±0.5)g的建鲤Cyprinus carpio var.Jian 49 d后,检测香菇多糖对幼建鲤主要肠道菌群数量及部分非特异性免疫指标的影响。结果表明:饲喂各试验剂量的香菇多糖均能极显著地提高幼建鲤的白细胞杀菌活性和血清中溶菌酶活性(P<0.01);饲喂1.6 g/kg(饲料)及以上剂量的香菇多糖,可使幼建鲤前、中、后肠中的乳酸杆菌、双歧杆菌数量较对照组极显著增加(P<0.01),且以2.4 g/kg(饲料)剂量组的刺激效果较佳;饲喂1.2 g/kg(饲料)及以上剂量的香菇多糖,可使幼建鲤前、中、后肠中的大肠杆菌、嗜水气单胞菌数量较对照组极显著减少(P<0.01),分别以2.0、2.4 g/kg(饲料)剂量组的刺激效果较佳。
The intestinal microflora and nonspeeific immune indexes were investigated in juvenile Jian common carp Cyprinus carpio vat. Jian with body weight of （40.0±0.5） g fed diets containing various levels of lentinan （0, 0.8, 1.2, 1.6, 2.0 ,and 2.4 g/kg diet） for 49 days. The results showed that the leukocyte bactericidal index and serum lysozyme activity were found very significant increase compared with the control group（ P〈0.01 ）. There were significantly more Lactobacilli and Bifidobacterium in the foregut, midgut and hindgut in the fish fed the diet contai- ning lentinan up to 1.6 g/kg diet compared with the fish in the control group, the maximum in the fish fed the diet containing lentinan at a dose of 2.4 g/kg diet. On the other hand, Escherichia coli and Aerornonas hydrophila in the foregut, midgut and hindgut was found to be significantly decreased（ P〈0.01 ）in number, with the optimal effective concentration of 2.0 g/kg, and 2.4 g/kg, respectively.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2013年第4期329-333,共5页
Journal of Dalian Ocean University
基金
四川省教育厅青年基金资助项目(09ZB055)
关键词
香菇多糖
建鲤
肠道菌群
非特异性免疫
白细胞杀菌活性
溶菌酶
lentinan
Cyprinus carpio var. Jian
intestinal microflora
nonspecific immunity
leucocyte bactericidal activity
lysozyme
