摘要
目的探讨Hedgehog信号通路与上皮-间质转化相关分子在进展期胃癌组织中的表达及其对预后的影响。方法采用免疫组化的方法检测121例进展期胃癌组织及对应正常胃黏膜组织中Gli-1(Hedgehog信号通路下游转录因子)及Snail、E-cadherin(上皮-间质转化相关因子)的表达情况,与临床病理资料及术后生存情况作对照分析。结果 (1)Gli-1、Snail及E-cadherin在胃癌组织中的阳性表达率分别为79.3%、76.0%、36.4%;在正常胃黏膜组织则为29.8%、29.8%、100%。3种蛋白在胃癌组织和正常胃黏膜组织中的表达差异均具有统计学意义(P<0.05)。(2)Gli-1的过度表达与肿瘤的浸润深度、有无淋巴结转移及pTNM分期有关(P<0.05);Snail的过度表达和E-cadherin的表达缺失则与组织学类型、浸润深度、淋巴结转移及pTNM分期有关(P<0.05)。(3)在胃癌组织中Gli-1的异常表达与Snail蛋白的表达呈正相关(P<0.05),与Ecadherin的表达呈负相关(P<0.05)。(4)Gli-1及Snail阳性表达的患者均较阴性患者表现出更差的预后和更低的生存率(P<0.05);而E-cadherin表达的患者则较不表达的患者表现出更好的预后(P<0.05)。E-cadherin的表达情况与组织学分类、有无淋巴结转移为影响胃癌患者预后的独立因素(P<0.05)。而Gli-1与Snail的表达情况则不是影响胃癌患者预后的独立因素(P>0.05)。结论在进展期胃癌中,Hedgehog信号通路的异常激活与上皮-间质转化的发生之间存在相关性,并且两者的发生对进展期胃癌患者的预后情况产生影响。
Objective To investigate the Hedgehog （Hh） signal pathway and Epithelial-mesenchymal transition （EMT） in advanced gastric cancer and the value of its clinical-pathological and prognosis significance.Methods Immunohistochemistry was used to detect the expression of Gli-1,Snail and E-cadherin proteins in 121 cases of advanced gastric cancer tissues and correspondingnormal gastric mucosa tissues.The correlation of Gli-1,Snail and E-cadherin expression with the clinicopathologicfeatures and prognostic survivals was analyzed.Results （1） The positive rate of Gli-1,Snail and E-cadherin expression was 79.3%,76.0% and 36.4% in GC tissues,29.8%,29.8% and 100% in corresponding normal gastric mucosa tissues,with a statistical significance between GC tissues and corresponding normal gastric mucosa tissues （P 〈 0.05）.（2） Excessive expression of Gli-1 was related to the depth of invasion,lymph node metastasis and pTNM staging （P 〈 0.05）.Snail excessive expression and E-cadherin lack expression were related tothe histological type,depth of invasion,lymph node metastasis and pTNM staging （P 〈 0.05）.（3） The expression of Gli-1 in GC was positively correlated with the expression of Snail （P 〈 0.05） and negatively correlated with the expression of E-cadherin （P 〈 0.05）.（4）The patients with either Gli-1 or Snail positive expression showed more poor prognosis and lower survival rate than the negative patients （P 〈 0.05）.While the patients with E-cadherin negative expression showed better after operation （P 〈 0.05）.Only the E-cadherin with histological type,with or without lymph node metastasis was the independent influencing factor of GC patients after operation （P 〈0.05）.Gli-1 and Snail were not independent influencing factors of GC patients after operation （P 〉 0.05）.Conclusion In advanced gastric cancer,the abnormal activation of Hedgehog signaling pathway has correlation with Epithelial-mesenchymal transition phenomenon happening.In addition,both have an effect on the prognosis in patients with advanced gastric cancer.
出处
《医学研究杂志》
2013年第9期119-124,共6页
Journal of Medical Research
