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Sphk1对SW480和HT-29体外血管生成拟态的影响 被引量：2

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摘要 目的:观察Sphk1对人结肠癌细胞体外生成VM的影响,并探讨其作用机制。方法:用100 nmol/L PMA处理HT-29、SW480作为2组激活组,用50μmol/L DMS处理SW480作为抑制组;在抑制组内分别加入rhMMP2、rhMMP9作为2组试验组。采用Matrigel三维培养法观察VM形成;MTT检测细胞生长增殖;Transwell观察细胞迁移;RT-PCR检测mRNA表达;Western blot、Elisa检测蛋白表达及分泌。结果:PMA上调Sphk1表达,促进VM表达阴性的HT-29体外形成VM,增强SW480增殖迁移能力,上调MMP2、MMP9的基因与蛋白表达分泌。DMS抑制Sphk1表达,减少SW480体外形成VM,加入rhMMP2、rhMMP9后再次形成VM,减弱SW480增殖迁移能力,下调MMP2、MMP9的基因与蛋白表达分泌。结论:Sphk1可促进结肠癌细胞形成VM,其机制可能通过增强结肠癌细胞的侵袭力并增加MMP2、MMP9的表达与分泌而发挥作用。
作者 李梦婷 黄杰安 周巧 苏颖洁 刘诗权
机构地区 广西医科大学第一附属医院消化内科
出处 《实用医学杂志》 CAS 北大核心 2013年第18期2967-2970,共4页 The Journal of Practical Medicine
基金 广西医疗卫生重点科研课题(编号:重2010016 GZKZ 10-107)
关键词 鞘氨醇激酶1 血管生成拟态 结肠癌细胞 金属基质酶
分类号 R735.35 [医药卫生—肿瘤]
  • 相关文献

参考文献12

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二级参考文献20

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共引文献8

同被引文献30

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二级引证文献5

实用医学杂志
2013年 第18期

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