摘要
为寻找新的高活性杀虫化合物,本研究以小菜蛾(Plutella xylostella)为靶标筛选出杀虫抗生素3704。室内生测试验表明:抗生素3704粗提物处理2龄小菜蛾幼虫24、48和72 h,LC50分别为59.82842、38.66165和22.69325μg/mL。盆栽试验表明:处理后48 h,500μg/mL的杀虫素3704粗提物对小菜2龄幼虫的防治效果与8μg/mL阿维菌素同期防效相当,但施药时间延长至72 h和96 h后,防治效果低于阿维菌素。分离纯化链霉菌 3704 杀虫活性物质,鉴定出其化学结构为已知杀螨剂哒螨酮(pyridaben),本研究首次报道从微生物次级代谢产物中分离出该化合物。
The objective of this study was to find new highly active insecticidal compounds. Antisect antibiotic 3704 was chosen using Plutella xylostella as a target. A laboratory bioassay was conducted on its insecticidal activity to the 2nd in- star larvae of P. xylostella. The values of LC50 were 59. 82842,38. 66165 and 22.69325μg/mL after treated with crude extract of 3704 for 24,48 and 72 h. A pot experiment indicated that after 48h treatment, the control efficacy of antisect antibiotic 3704 against P. xylosteUa at 500 μg/mL was equal to that of Avermectin at 8 μg/mL. When the treatment time was longer to 72h and 96 h,the control efficacy of antisect antibiotic 3704 was lower than that of Avermectin. The insec- ticidal compound was isolated, purified and identified as Pyridaben. To our knowledge, this is the first report of isolating Pyridaben from microbiological metabolites.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第9期1172-1174,共3页
Natural Product Research and Development
基金
国家自然科学基金项目(30971958)
关键词
链霉菌3704
杀虫活性
小菜蛾
哒螨酮
streptomyces 3704
insecticidal activity
Plutella xylostella
Pyridaben
