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PCR-DGGE技术在废水生物处理中的应用研究 被引量:3

PCR-DGGE Technique in Biological Wastewater Treatment Application
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摘要 聚合酶链式反应-变形梯度凝胶电泳(PCR-DGGE)作为一种分子指纹图谱能够较准确地反应污水处理过程中微生物群落结构多样性及其动态变化而广泛应用于废水生物处理技术的研究中。PCR-DGGE技术不仅能对样品中微生物群落结构多样性及种群演替进行分析,还能用于污水处理中优势菌群的分离与鉴定,克服了传统方法费时费力、培养条件苛刻等局限性,进而运用该技术构建高效的污染物降解工程菌,提高难降解有机物的去除效率,调节实际工艺参数,提高污水处理效率。 Polymerase Chain Reaction - Deformation Gradient Gel Electrophoresis ( PCR - DGGE) as a molecular finger- print can more accurately reflect the sewage treatment process microbial community structure and dynamics and diversity are wide- ly used in biological wastewater treatment. PCR - DGGE technique can not only diversity of microbial community structure in the sample and population succession analysis, but also for sewage treatment isolation and identification of advantage bacteria to over- come the traditional method is time consuming and laborious, culture conditions such as harsh limitations Furthermore the use of the technology to build efficient degradation of pollutants engineered bacteria to improve the efficiency of refractory organics remov- al, adjust the actual process parameters to improve the efficiency of wastewater treatment.
出处 《环境科学与管理》 CAS 2013年第9期115-117,136,共4页 Environmental Science and Management
关键词 PCR—DGGE 废水 生物处理 微生物 PCR - DGGE waste water biological treatment microorganism
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  • 1His -Jien Chen, Yi -Zi Lin ,Jen- Mao Fanjiang, Chih- hao Fan. Microbial community and treatment ability investigation in AOAO process for the optoelectronic wastewater treatment u- sing PCR - DGGE biotechnology [ J ]. Biodegradation, 2013,24 :227 - 243.
  • 2Ruoyu Chert, Timothy M. LaPara. Aerobic Biological Treatment of Low - Strength Synthetic W - astewater in Mem- brane - Coupled Bioreactors :The Structure and Function of Bac- terial Enrich -ment Cultures as the Net Growth Rate Approaches Zero[ J]. Microbial Ecology,2006,51:99 - 108.
  • 3Irena Wojnowska - Baryla,Agnieszka Cydzik - Kwiatkows- ka, Magdalena Zielifiska. The Applica - tion of Molecular Tech- niques to the Study of Wastewater Treatment Systems[ J ]. Bioremed- iati - on,2010,599:157 - 183.
  • 4Timothy M. LaPara, Cindy H. Nakatsu, Lisa M. Pantea. Stability of the bacterial communities supported by a seven - stage biological process treating pharmaceutical wastewater as revealed by PCR - DGGE [ J ]. Water Research ,2002,36 (3) :638 - 646.
  • 5Aure1ie Cebmn,Manuela Coci ,Josette Gamier, Hendrikus J. Laanbroek. Denaturing Gradient Gel Electrophoretic Analysis of Ammonia- Oxidizing Bacterial Community Structure in the lower Seine River: Impact of Paris Wastewater Effluents[J]. Applied and Environmental Microbiology,2004( 11 ) :6726 -6737.
  • 6沈丽尧,冷云伟,何环,金德才,韩娅新,王珊珊.酿造废水处理系统中微生物多样性的分析[J].中国酿造,2012,31(10):162-165. 被引量:7
  • 7陈建中,李钧敏,余彬彬,王岳,徐丽丽.医化废水处理池活性污泥微生物多样性分析[J].江苏农业科学,2010,38(5):454-456. 被引量:1
  • 8Calli B, Mertoglu B, Roest K, et al. Comparison of long - term performances and final microb - ial compositions of anae- robic reactors treating landfill leachate [ J ]. Bioresource Technol- ogy,2006,97 (4) :641 - 647.
  • 9Abd El-Latif Hesham,Rong Qi,Min Yang.Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis[J].Journal of Environmental Sciences,2011,23(12):2049-2054. 被引量:26
  • 10许玫英,曾国驱,任随周,岑英华,孙国萍,郭俊.分子检测技术对活性污泥中氨氧化细菌的比较研究[J].微生物学报,2003,43(3):372-378. 被引量:19

二级参考文献94

共引文献148

同被引文献20

  • 1王建芳,赵庆良,林佶侃,金文标.生物强化技术及其在废水生物处理中的应用[J].环境工程学报,2007,1(9):40-45. 被引量:54
  • 2HJ/T 399-2007 水质化学需氧量的测定快速消解分光光度法[S].
  • 3Mohajeri S, Aziz H A, isa M H. Statistical optimiza- tion of process parameters for landfill leachate treatment using e- lectro - Fenton technique [ J ]. Journal of Hazardous Materials, 2,010(176) :749 - 758.
  • 4Krzemieniewski M, Debowski M, Dobrzynska A, et al. Chemical oxygen demand reduction of various wastewater types using magnetic field-assisted Fenton reaction [ J ]. Water Environment Research, 2004, 76 (4): 301 -309.
  • 5Papadimitriou C A, Samaras P, Sakellaropoulos G P. Comparative study of phenol and cyanide containing wastewater in CSTR and SBR activated sludge reactors [ J]. Bioresource Technology, 2009, 100 (1): 31 -37.
  • 6Wang J L, Quan X C, Wu L B, et al. Bioaugmentation as a tool to enhance the removal of refractory compound in coke plant wastewater [ J]. Process Biochemistry, 2002, 38 (5) : 777 - 781.
  • 7Murray A E, Hollibaugh J T, Orrego C. Phylogeneticcompositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments [J]. Applied and Environmental Microbiology, 1996, 62 (7) : 2676 - 2680.
  • 8Donner G, Schwarz K, Hoppe H G, et al. Profiling the succession of bacterial populations in pelagic chemoclines [J]. Ergebnisse der Limnologie, 1996, 48 : 7 - 14.
  • 9Muyzer G, De Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA I J]. Applied and Environmental Microbiology, 1993, 59 (3) : 695 -700.
  • 10毛庚仁,张涌新,文雯,何董瑞.我国城市生活垃圾处理现状及焚烧法的可行性分析[J].城市发展研究,2010,17(9). 被引量:33

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