摘要
目的为研究筋膜中结缔组织在热敏灸信号转导中的作用,揭示热敏灸产生疗效的有关机制,为临床更好应用热敏灸提供相关理论支持。方法选取80只SD雌性大鼠随机分为正常组,空白模型组、艾灸模型组及热敏灸模型组各20只,并对后三组进行造模使其处于骨质疏松症病理状态下,然后对艾灸模型组及热敏灸模型组取"肾俞"穴进行常规艾灸及热敏灸治疗,并取其灸点周围结缔组织进行染色,从形态学上及光密度值上观察各组筋膜中ERK1/2和P38的改变情况,并进行统计学分析。结果正常组与空白模型组中ERK1/2和P38改变不大,艾灸与热敏灸模型组中ERK1/2和P38表达情况都有不同程度改变,尤其热敏灸模型组改变情况明显,ERK1/2阳性染色区域多集中在成纤维细胞或巨噬细胞核周围;P38在筋膜中的表达稍弱,表达阳性区域也多集中筋膜中成纤维细胞或巨噬细胞核周围。定量分析其差异有显著性意义。结论实施热敏灸时,灸信号通过使热敏态穴区筋膜结缔组织中MAPK信号通路蛋白发生变化,进而对人体生理病理状态起调节作用。
Objective To investigate the effect of fascial connective tissue in the thermal moxibustion signal transduction, and to reveal the relevant mechanism of the thermal moxibustion, in order to provide relevant theory basis for the better use of thermal moxibustion in clinical work. Methods Eighty female SD rats were randomly divided into normal group, model group, moxibustion model group, and thermal moxibustion model group. Each group had 20 rats. Osteoporosis model was established in the latter 3 groups. Rat inmoxibustion model group and thermal moxibustion model group were given the conventional moxibustion or thermal moxibustion treatment in the Shenshu point. The connective tissue around the point was collected for staining, and the changes of ERK1/2 and P38 in the fascia were observed by morphology and optimal density. The data were statistically analyzed. Results The expression of ERK1/2 and P38 in normal group and the blank model group changed very little. In the moxibustion model group and thermal moxibustion model group, the expression of ERK1/2 and P38 changed in varying degree. Especially in thermal moxibustion model group, the ERK1/2 postive staining area mostly located around the nucleus of fibroblasts and macrophages. The expression of P38 was slightly weak, and the postive staining area also mostly located around the nucleus of fibroblasts and macrophages. The quantitive analysis showed the variance of expression had significant difference. Conclusion In the process of thermal moxibustion, the moxibustion signal can promote the changes of MAPK signaling pathway proteins in fascial connective tissue around the moxibustion area, thus regulating the physiological and pathological status.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2013年第9期919-921,940,共4页
Chinese Journal of Osteoporosis
基金
江西省青年基金项目(20122BAB215047)
江西省卫生厅中医药科研基金项目(2012A055)
