摘要
目的:探讨HOXA10和HOXA11基因对子宫内膜异位(endometriosis,EMs)不孕症患者内膜组织容受性的影响。方法:应用荧光定量RT-PCR和蛋白印迹法分别检测EMs不孕症患者(n=23,EMs组)和具有正常生育能力女性(n=20,对照组)分泌期子宫内膜组织中HOXA10和HOXA11 mRNA及蛋白的表达水平。结果:HOXA10和HOXA11基因在EMs组和对照组分泌期子宫内膜中均有表达。EMs组HOXA10和HOXA11 mRNA和蛋白的表达显著低于对照组(P<0.05)。结论:EMs不孕症组子宫内膜组织中HOXA10和HOXA11基因的表达水平显著下调,提示EMs可能通过影响子宫内膜组织中HOXA10和HOXA11基因的正常表达而干扰子宫内膜容受性的形成,从而导致胚胎着床失败和不孕。
Objective： To investigate the clinical significance for endometrial receptivity of HOXAIO and HOXA11 gene in the infertility patients of endometriosis （EMs）. Methods： The mRNA and protein expression levels of HOXA10 and HOXA11 in secretory phase tissues of EMs-associated infertility group （n=23, EMs group） and healthy women group （n=20, control group） were detected by FQ-PCR and Western blotting. Results： HOXAIO and HOXA11 genes were expressed in the two groups. The expression levels ofHOXAIO mRNA and protein were lower in EMs group than those in the control （P〈0.01）. The expression levels ofHOXA11 mRNA and protein in EMs group were also lower than those in the control （P〈0.05）. Conclusion： The expression of gene HOXAIO and HOXA11 on both nucleic acid and protein levels are lower in infertile EMs patients than in the control. The lower expression of gene HOXAIO and HOXA11 may be involved in the infertility of EMs patients by reducing endometrial receptivity which cause embryo implantation failure.
出处
《生殖与避孕》
CAS
CSCD
2013年第9期586-590,共5页
Reproduction and Contraception
基金
湖北省自然基金
项目编号:2009CDB168
