摘要
目的 探讨血必净对鲍曼不动杆菌脓毒症大鼠肝脏组织翻译控制肿瘤蛋白（TCTP）表达的影响.方法 健康清洁级成年雄性Wistar大鼠42只,随机取6只大鼠作为对照组；余36只按随机数字表法均分为脓毒症组和血必净组.通过腹腔注射鲍曼不动杆菌悬液制作脓毒症模型,30 min后血必净组尾静脉注射血必净注射液.脓毒症组和血必净组于制模后6、12和24 h随机取6只大鼠,处死取肝脏组织标本,苏木素-伊红（HE）染色,观察肝脏组织病理学改变；免疫组化法分析肝脏组织TCTP表达阳性细胞；蛋白质免疫印迹试验（Western blotting）检测肝细胞TCTP的表达.结果 HE染色显示,脓毒症组肝脏炎性受损,血必净组肝组织炎性损伤较脓毒症组减轻.免疫组化结果显示,与对照组比较,脓毒症组6、12和24 h TCTP阳性细胞表达评分（分）明显升高（7.33 ± 0.82、10.67±1.21、7.67±1.21比2.50±1.05,均P＜0.05）；与脓毒症组比较,血必净组6、12和24 h肝组织TCTP阳性细胞表达评分（5.83±0.75、7.50±1.05、5.67±1.37）均降低（均P＜ 0.05）.Western blotting结果显示,与对照组比较,脓毒症组6、12和24hTCTP表达量明显增加（1.94±0.59、3.20±0.72、1.96±0.55比0.93±0.24,均P＜0.05）；与脓毒症组比较,血必净组6、12和24 h TCTP表达量（1.38±0.36、2.03±0.49、1.30±0.30）明显降低（均P＜0.05）.结论 血必净有利于减轻鲍曼不动杆菌脓毒症大鼠肝脏的炎性损伤,其机制可能与降低肝细胞表达TCTP有关.
Objective To study the effect of Xuebijing on liver expressing translationally controlled tumor protein （TCTP） in Acinetobacter baumannii sepsis rats.Methods Among 42 healthy adult male Wistar rats of clean grade,6 rats were randomly selected as the control group,others were randomly divided into two groups by the method of random digits table：sepsis group （n =18）,Xuebijing group （n =18）.Sepsis model was established through intraperitoneal injecting Acinetobacter baumannii suspension,and the Xuebijing injection was administrated through caudal vein 30 minutes later in Xuebijing group.After making model for 6,12 and 24 hours,6 rats were randomly selected from sepsis group and Xuebijing group,and then the rats were sacrificed,liver tissue samples were extracted for hematoxylin and eosin （HE） staining.Pathological changes of the liver were observed,and immunohistochemical analysis of liver tissue TCTP expression positive cells and the expression of TCTP in liver cells were detected by Western blotting method.Results HE staining of liver indicated that it was inflammatory injured in sepsis group,and inflammation decreased in Xuebijing group.Immunohistochemistry results showed that,compared with the control group,TCTP positive cells expression score at 6,12 and 24 hours in sepsis group were significantly increased （7.33 ± 0.82,10.67 ± 1.21,7.67 ± 1.21 vs.2.50 ± 1.05,all P〈0.05）.Compared with sepsis group,liver tissue TCTP positive cells expression score at 6,12 and 24 hours in Xuebijing group （5.83 ± 0.75,7.50 ± 1.05,5.67 ± 1.37） were significantly decreased （all P〈0.05）.Western blotting results showed that,compared with the control group,TCTP expression at 6,12 and 24 hours in sepsis group were significantly increased （1.94 ±0.59,3.20 ±0.72,1.96 ±0.55 vs.0.93 ±0.24,all P〈0.05）; compared with sepsis group,TCTP expression at 6,12 and 24 hours in Xuebijing group （1.38 ± 0.36,2.03 ± 0.49,1.30 ± 0.30） were significantly decreased （all P〈0.05）.Conclusion Xuebijing can reduce inflammatory injury in liver of rats with Acinetobacter baumannii sepsis,and its mechanism may be associated with reduced hepatic cells expressed TCTP.
出处
《中华危重病急救医学》
CAS
CSCD
北大核心
2013年第9期537-541,共5页
Chinese Critical Care Medicine
基金
安徽省高校省级自然科学研究重点项目（KJ2013A186）
关键词
血必净
脓毒症
鲍曼不动杆菌
翻译控制肿瘤蛋白
肝脏
Xuebijing
Sepsis
Aeinetobacter baumannii
Translationally controlled tumor protein
Liver
