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HPLC测定降粘胶囊中绿原酸的含量 被引量:1

HPLC Determination of Chlorogenic Acid in Jiangnian Capsules
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摘要 目的:建立测定降粘胶囊中绿原酸含量的高效液相色谱法。方法:采用Phenomenex C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.2%磷酸(20∶80),流速1.0 mL·min-1,检测波长327 nm,进样量10μL,柱温30℃。结果:绿原酸质量浓度在4.344~130.3 mg·L-1(r=0.999 8)与峰面积呈良好线性关系,平均加样回收率为99.93%(RSD 0.12%)。结论:方法测定降粘胶囊中绿原酸的含量,简便、准确,结果稳定,可为降粘胶囊的质量评价提供依据。 Objective: To determine the content of chlorogenic acid in Jiangnian Capsules by HPLC.Method: Phenomenex C 18 column(4.6 mm × 250 mm,5 μm) was used.The mobile phase was methanol-0.2% phosphoric acid(20∶ 80) and the detecting wavelength was set at 327 nm;the flow rate was 1.0 mL·min-1,and the injection volume was 10 μL;the column temperature was kept at 30 ℃.Result: The linear range of calibration curve was 4.344-130.3 mg·L-1for chlorogenic acid(r = 0.999 8).The average recovery rate was 99.93%(RSD 0.12%).Conclusion: The method is highly sensitive,quick and accurate with good repeatability,which can be used for the quality control of Jiangnian Capsules.
出处 《中国实验方剂学杂志》 CAS 北大核心 2013年第18期148-150,共3页 Chinese Journal of Experimental Traditional Medical Formulae
关键词 降粘胶囊 绿原酸 高效液相色谱 Jiangnian Capsules chlorogenic acid HPLC
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