摘要
目的:研究炒制对细辛中黄樟醚、甲基丁香酚和细辛脂素含量的影响。方法:采用HPLC,Agilent Eclipse XDBC18色谱柱(4.6 mm×150 mm,5μm),流动相甲醇-水,梯度洗脱,流速1.0 mL·min-1,检测波长285 nm,柱温35℃。结果:甲基丁香酚、黄樟醚和细辛脂素为进样量分别在0.103 7～2.074μg(r=0.999 9),0.110 1～2.02μg(r=0.999 9),0.054 3～1.086μg(r=0.999 9)有良好的线性关系,平均回收率分别为98.74%(RSD 2.10%),96.93%(RSD 1.58%),101.92%(RSD3.55%)。结论:细辛炒制可显著降低毒性成分黄樟醚的含量,较好地保留有效成分甲基丁香酚和细辛脂素,起到减毒存效的作用。因此,细辛炒制具有一定的合理性。
Objective： To analyze the content changes of safrole, methyleugenol and asarinin by different processing methods in Asari Radix et Rhizoma. Method： Safrole, methyleugenol and asarinin were determined by Agilent Eclipse XDB-C,s （4.6 mm x 150 ram, 5 μm） column as stationary phase, 285 nm as detective wavelength, MeOH-H20 as mobile phase; the flow rate was 1.0 mL .min-1; the column temperature was kept at 35 ℃. Result： The linear ranges of methyleugenol, safrole and asarinin were 0. 103 7-2. 074 μg （r =0. 999 9） , 0. 110 1-2. 202 μg （ r = 0. 999 9）, 0. 054 3-1. 086 μg （ r = 0. 999 9） and the average recoveries were 98.74% , 96.93% , 101.92% and RSDs were 2. 10% , 1.58% , 3.55% respectively. Conclusion： The content of safrole decreased signigicantly in stri-fied produts, while the content of methyleugentol and asarinin was conserved effectively. Therefore, it is very sensible to stir-fire Asari Radix et Rhizoma.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第19期90-92,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点基础研究发展计划(973计划)项目(2009CB522804)
