摘要
目的:考察覆盆子有效部位对D-半乳糖联合氢化可的松造成肾阳虚型痴呆大鼠的影响,初步阐明覆盆子有效部位改善肾阳虚型痴呆大鼠学习记忆的作用机制。方法:大鼠ip D-半乳糖125 mg·kg-1,连续6周,后2周im氢化可的松混悬液25 mg·kg-1,造成肾阳虚痴呆模型,空白组和模型组灌胃生理盐水,其余各组每天均预防性灌胃覆盆子氯仿部位或乙酸乙酯部位高、低剂量(均按生药量计为24,12 g·kg-1),连续4周。进行历时5 d的Morris水迷宫实验后,取大鼠皮层进行乙酰胆碱酯酶(acetylcholinesterase,AchE)和胆碱乙酰化转移酶(choline acyltransferase,ChAT)活性检测、取海马CA1区进行HE染色和tau蛋白免疫组织化学检测。结果:与空白组相比,模型组学习记忆能力下降,皮层AchE活性显著升高,ChAT活性显著降低,海马CA1区Pser404-tau阳性细胞明显增加;与模型组比较,覆盆子氯仿部位高、低剂量组、覆盆子乙酸乙酯部位高、低剂量组均能不同程度改善痴呆大鼠学习记忆能力,降低皮层AchE活性,升高ChAT活性;增加海马CA1区细胞总数,减少坏死细胞数,降低细胞坏死率;减少海马CA1区Pser404-tau阳性细胞数。结论:覆盆子有效部位可能通过降低AchE活性,升高ChAT活性,保护海马CA1区神经元,减少tau蛋白表达而起到改善肾阳虚型痴呆大鼠学习记忆。
Objective： To explore the impact of Rubus chingii Hu＇s effective parts on kidney-yang deficiency Alzheimer＇s disease （AD） rats caused by D-galactose combined with hydrocortisone, and clarify the active ingredient Of raspberry in anti-kidney-yang deficiency AD. Method： Rats were injected intraperitoneally with D-galactose in dose of 125 mg·kg^-1 for six weeks. All rats were intramuscularly injected with hydrocortisone 25 mg ·kg^-1 for the last two weeks to establish the rat kidney-yang deficiency AD model. The blank group and the model group were given with saline, other groups were administrated with R. chingii high-dose group （24 g ·kg^-1 ） , R. chingii low-dose group （12 g ·kg^-1 ）, ethyl acetate high-dose group （24 g·kg^-1 ） and ethyl acetate low dose-group （12 g ·kg^-1 ）for 4 weeks.＇ After Morris water maze test, acetylcholinesterase （AchE） and choline acyltransferase （CHAT） activity of the cortex was detected. The hippocampal CA1 region for HE staining was observed and tau protein content was assayed with immunohistochemical method. Result： The R. chingii high-dose group, R. chingii low-dose group, ethyl acetate high dose group and ethyl acetate low dose groupcould improve the learning and memory abilities reduce cortex AchE activity and increase ChAT activity. -The total numbers of cells in the hippocampus CA1 area were increased, the necrotic cells and the cell necrosis rat were decreased. Pser404-tau positive cells of the hippocampus CA1 area were decreased. Conclusion： R. chingii effective parts can improve learning and memory abilities of kidney-yang deficiency AD rats by reducing AchE activity, increasing ChAT activity, protecting neurons of hippocampal CA1 area and decreasing tau protein expression.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第19期192-196,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金课题(30960496)
关键词
覆盆子
痴呆
胆碱能功能
神经元保护
TAU蛋白磷酸化
Rubus chingii
dementia
choline function
neuronprotection
tau protein phosphorylation
