摘要
目的探讨大柴胡汤防治动脉粥样硬化(AS)性疾病的可能作用机制。方法 50只家兔随机分为空白组、模型组、血脂康组、预防组及治疗组,每组10只。采用家兔给予高脂饲料6周建立AS动物模型,空白组和模型组给予生理盐水20ml灌胃;血脂康组给予血脂康20ml灌胃(生药量0.8g);预防组给予大柴胡汤20ml(生药量7.5g)灌胃,以上每组第1周开始灌胃,均每日1次,连续10周;治疗组第1周开始给予生理盐水20ml灌胃,每日1次,连续6周,第7周开始给予大柴胡汤20ml(生药量7.5g)灌胃,每日1次,连续4周。取出主动脉于光镜下进行病理观察,检测各组大鼠血脂、C反应蛋白(CRP)、白细胞介素8(IL-8)mRNA和蛋白含量。结果病理结果显示,预防组和治疗组家兔斑块面积及厚度较模型组小,泡沫细胞数量及炎症细胞浸润较少,斑块内未见坏死和瘢痕。血脂康组、预防组和治疗组血脂水平、CRP含量、IL-8mRNA及蛋白表达显著低于模型组(P<0.01),预防组在降低血脂方面优于治疗组(P<0.01),预防组在降低CRP水平、IL-8mRNA及蛋白表达方面优于血脂康组(P<0.05或P<0.01)。结论大柴胡汤具有明显的降低血脂的作用,并可能通过抑制家兔IL-8的表达、减少炎症细胞浸润,从而起到延缓AS斑块形成的作用。
Objective To research the possible mechanism of Dachaihu Decoction （Major Bupleurum Decoction） for atherosclerosis（AS）. Methods Fifty rabbits were randomized into the blank group, model group, Xuezhikang group, prevention group and treatment group, with 10 in each. The AS rabbit models were established by giving high fat diet for 6 weeks. The blank group and model group were gavaged with 20 ml normal saline （NS）. The Xuezhikang group was gavaged with 20 ml Xuezhikang （0.8g crude drug）. The prevention group was gavaged with 20 ml Dachaihu Decoction （7.5 g crude drug）. Each group mentioned above was given intragastric administration once daily from the 1st week to the 10th week. The treatment group was gavaged with 20ml NS once daily from the 1st week to the 6th week and 20ml Dachaihu Decoction （7. 5 g crude drug） once daily from the 7th week to the 10th week. The aortic pathology was observed with light microscope. The serum lipid levels, C-reactive protein （CRP） contents and interleukin-8 （IL-8）mRNA expression were detected. Results Pathology showed the plaque area and thickness in the prevention group and treatment group were smaller than those in the model group and with less foam cells and inflammatory cell infiltration and no necrosis and scarring in the plaque. Comparing with the model group, the serum lipid levels, CRP contents and IL-SmRNA expression were significantly decreased in the Xuezhikang group, prevention group and treatment group （P〈0.01）. The prevention group was superior to the treatment group in reducing serum lipid （P〈0.01）. The prevention group was superior to the Xuezhikang group in reducing CRP level and IL-SmRNA expression （P〈0.05 or P〈0.01）. Conclusion Dachaihu Decoction can obviously decrease serum lipid and delay plaque formation by inhibiting IL-8 expression and reducing inflammatory cell infiltration.
出处
《中医杂志》
CSCD
北大核心
2013年第19期1681-1685,共5页
Journal of Traditional Chinese Medicine
