摘要
目的:采用高效液相色谱法测定抗病毒口服液中(R,S)-告依春含量的方法。方法:色谱条件:Phenomenex C_(18)柱(4.6 mm×250 mm,5μm);流动相:甲醇-0.02%磷酸溶液(7:93);流速:1 mL·min^(-1);柱温:30℃;检测波长:245 nm。结果:甘草酸铵在进样量0.017~0.107μg范围内线性关系良好,相关系数r=0.9996,回收率(n=6)为99.0%,RSD为1.26%。结论:本法准确可靠,重现性好,可用于测定抗病毒口服液中(R,S)-告依春的含量。
Objective ： To establish an HPLC method for the determination of epigoitrin in antivirus oral medicinal liquid. Methods ： The chromatographic separation was performed on Phenomenex Ci8 column （ 4.6 mm× 250 mm, 5 μ m ）, mobile phase was methanol-0.02% phosphoric acid （ 7 ： 93 ）, the flow rate was 1 mL · min-1 at 30 OC and the detection wavelength was 245 nm. Result： The results showed that the linearity for epigoitrin was in a range of 0.017-0.107 p~ g （ r = 0.9996 ）, and the average recovery and RSD were 99.0% and 1.26% respectively （ n=6 ） .Conclusion ： The method is accurate, reliable and reproducible, and could be used as a quality control method for epigoitrin in antivirus oral medicinal liquid.
出处
《辽宁中医药大学学报》
CAS
2013年第10期51-52,共2页
Journal of Liaoning University of Traditional Chinese Medicine
