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海马腺苷酸活化蛋白激酶水平与老龄大鼠脾切除术后认知功能障碍的关系 被引量：6

Relationship between level of AMP-activated protein kinase in hippocampus and cognitive dysfunction after splenectomy in aged rats
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摘要 目的 评价海马腺苷酸活化蛋白激酶（AMPK）水平与老龄大鼠脾切除术后认知功能障碍（POCD）的关系.方法 健康雄性老龄SD大鼠63只,体重480～550 g,采用随机数字表法,将其分为3组（n=21）：对照组（C组）、麻醉组（A组）和手术组（S组）.S组于术前、术后1、3、7d、A组和C组于相应时点进行Morris水迷宫实验,记录逃避潜伏期和游泳距离.术后1、3、7d水迷宫实验结束后随机取7只大鼠,处死后取海马组织,采用Western blot法测定海马AMPK、磷酸化AMPK（p-AMPK）的表达水平.结果 与术前相比,S组术后1、3d逃避潜伏期和游泳距离延长（P＜0.05）；与C组相比,S组术后1、3d逃避潜伏期和游泳距离延长,术后1、3、7d海马AMPK表达上调,术后1、3 d p-AMPK表达上调（P＜0.05）,A组上述指标差异无统计学意义（P＞0.05）.结论 海马AMPK水平升高是老龄大鼠脾切除术后认知功能障碍形成时机体的适应性调节机制. Objective To evaluate the relationship between the level of AMP-activated protein kinase （AMPK） in hippocampus and cognitive dysfunction after splenectomy in aged rats.Methods Sixty-three male aged Sprague-Dawley rats,weighing 480-550 g,were randomly divided into 3 groups （n =21 each）：control group （group C）,anesthesia group （group A）,and surgery group （group S）.Morris water maze （MWM） test was performed to evaluate the spatial learning and memory ability before surgery and at 1,3 and 7 days after surgery.The escape latency and swimming distance were recorded.Seven rats were chosen after MWM test was performed at 1,3 and 7 days after surgery and sacrificed.Their hippocampi were removed for detection of the expression of AMPK and phosphorylated （p-AMPK） （by Western blot）.Results Compared with the baseline,the escape latency and swimming distance were significantly prolonged at 1 and 3 days after surgery in group S （P ＜ 0.05）.Compared with group C,the escape latency and swimming distance were significantly prolonged at 1 and 3 days after surgery,the expression of AMPK was up-regulated at 1,3 and 7 days after surgery,and the expression of p-AMPK was up-regulated at 1 and 3 days after surgery in group S （P ＜ 0.05）,and no significant changes were found in the indices mentioned above in group A （P ＞ 0.05）.Conclusion Increased AMPK level in hippocampus is the regulatory mechanism of the body adapting to the development of cognitive dysfunction after splenectomy in aged rats.
作者 刘淑云 丛丽 刘湘萍 于春锐 万效梅 王明山 毕燕琳
机构地区 潍坊医学院青岛市市立医院东院区麻醉科
出处 《中华麻醉学杂志》 CAS CSCD 北大核心 2013年第7期832-834,共3页 Chinese Journal of Anesthesiology
基金 青岛科技局立项课题（10-3-3-4-8-nsh）
关键词 蛋白质丝氨酸苏氨酸激酶 认知障碍 手术后并发症 老年人 海马 Protein-serine-threonine kinases Cognition disorders Postoperative complications Aged Hippocampus
分类号 R749.2 [医药卫生—神经病学与精神病学]
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参考文献12

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共引文献14

同被引文献57

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中华麻醉学杂志
2013年 第7期

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