摘要
目的：从酒糟中提取多糖，并建立一种操作简单、结果可靠的检测酒糟多糖含量的方法。方法：采用酶法提取酒糟多糖，用a-萘酚-硫酸显色法测定多糖含量。结果：溶液最大吸收波长为560nm，在6-36gg／mL范围内吸光度与被测物含量呈良好的线性关系，R2=：0．9975，平均加标回收率100．65％，以葡萄糖计，酒糟中多糖含量为10．6％，RSD为4．0％。结论：a-萘酚-硫酸显色法测定酒糟多糖含量步骤简单，重复性好，结果可靠。
Objective： To extract polysaccharides from vinasse （byproduct of Chinese liquor making） and to establish a simple and reliable determination method. Methods： Vinasse polysaccharides were extracted by an enzymatic method, and a-naphthol-sulfuric acid method was used for the determination of polysaccharide content. Results： The maximum absorption wavelength was 560 nm. There was a good linear relationship between absorbance and glucose concentration in the range from 6 to 36 μg/mL （R2 = 0.9975）. The average spiked recovery rate was 100.65%. The content ofpolysaccharides from vinasse （caculated as glucose） was 10.6% （RSD = 4.0%）. Conclusion： The a-naphthol-sulfuric acid method is a simple and reliable assay with good reproducibility for determining polysaccharides from vinasse.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第18期245-248,共4页
Food Science
基金
遵义市科技局资助项目(遵市科合社字[2010]12号)
遵义师范学院区域经济中心资助项目(ZE201022)
关键词
酒糟
多糖
α-萘酚-硫酸法
vinasse polysaccharides
a-naphthol-sulfuric acid method
