摘要
首次提出Cu+-H2 O2 -茜素紫分析新体系并用于羟自由基的测定。本方法用Cu+催化H2 O2 反应产生·OH ;加入茜素紫显色剂使茜素紫的颜色发生变化 ,用紫外 -分光光度计测其ΔA值的变化 ,可间接测定羟自由基的产生量。通过测定条件的研究 ,得出最佳实验条件。结果表明 ,该方法稳定性好 ,操作简便 ,测定快速 。
Hydroxyl radical produced by Cu +/ H 2O 2 reaction was assayed by the new method,after oxidized by hydroxyl radical, the A 560 of alizarin violet decreased apparently. The ΔA 560 was department upon the dosage of alizarin violet,CuCl or H 2O 2 individually. The A 560 increased as the reaction lasted. It was demonstrated that the hydroxyl radical scanvenge effect of mannitol, benzoic acid,and thiourea was dosedependent by the new mothod.
出处
《应用化工》
CAS
CSCD
2000年第4期30-31,37,共3页
Applied Chemical Industry
基金
国家自然科学基金重点资助项目(29773012)
