摘要
目的:研究周围神经内部显微结构的显微拉曼光谱和超光谱成像特征。方法:取新西兰大白兔10只,解剖并获取其骶1脊髓前根和后根标本,随机选取各10例标本作厚度为30μm冷冻切片。用波长633 nm的激光扫描神经纤维断面;随机各取3例前根和后根标本作厚度为10μm冷冻切片,用超光谱成像系统采集标本的超光谱数据。每个断面对轴突和髓鞘各取15个感兴趣区域(regions of interest,ROI)进行分析。结果:周围神经纤维的拉曼光谱在550 cm-1、1080 cm-1、1280 cm-1、1440cm-1、1660 cm-1处有明显的拉曼散射,以1440 cm-1处最为显著;运动和感觉神经纤维的拉曼光谱相似;周围神经髓鞘与轴突的超光谱曲线有区别。结论:周围神经感觉与运动神经纤维的显微拉曼光谱和超光谱曲线相似,难以独立进行区分。超光谱成像技术可直接对周围神经髓鞘进行识别。
Objective：To explore the characteristics of the microscopic structures in peripheral nerves under Raman spectroscopy and hyperspectral imaging.Methods：S1 anterior and posterior roots were collected from ten sacrificed New Zealand rabbits.Ten randomly selected anterior and posterior roots were frozen sectioned at 30 μm thickness and scanned under Raman micro-spectroscopy with a 633 nm laser.Another three randomly selected anterior and posterior roots were frozen sectioned at 10 μm thickness and processed with hyperspectral imaging.Fifteen regions of interest（ROI） were marked in each image to collect their spectra for analysis.Results： Significant Raman scattering were detected at 550 cm-1,1080 cm-1,1280 cm-1,1440 cm-1,1660 cm-1 in the Raman spectra of peripheral nerve fascicles,and 1440 cm-1 was of the greatest value.Raman spectra between motor and sensory fascicles had no significant difference.Raman spectra of peripheral nerve motor and sensory fascicles could not be distinguished.Hyperspectra of myelin sheaths were significantly different from that of axons.Conclusions： Raman spectra and hyperspectra of peripheral nerve motor and sensory fascicles are similar and cannot be differentiated independently.Hyperspectral imaging technology can directly distinguish myelin sheaths in peripheral nerve sections.
出处
《中国临床医学》
2013年第3期245-248,共4页
Chinese Journal of Clinical Medicine
基金
2010年上海市科委重点攻关项目计划(编号:10411953900)
关键词
周围神经
髓鞘
拉曼光谱
超光谱成像
Peripheral nerve
Myelin sheath
Raman spectroscopy
Hyperspectral imaging
