摘要
建立了一种快速检测腐竹中鸟洛托品的高效液相色谱方法。确定的实验条件为：利用氯仿提取样品，采用shim．DackvP-oDs柱（4．6mm×150mm，5pm），柱温30℃，检测波长210nm采用甲醇水（体积比为75：25）为流动相，流速0．5mL／min。在确定的实验条件下，乌洛托品在10—500mg／L范围内线性关系良好，相关系数，=0．9991，平均回收率为96．45％，RSD=b1．60％。本方法灵敏度高，结果准确、可靠，可用于腐竹中乌洛托品的含量检测。
A rapid detection of urotopine in yuba by high performance liquid chromatography has been established with the following experimental conditions： chloroform is used in sample extraction, Shim-pack VP-ODS column 4.6 mm X 150 mm, column temperature 30 ℃, the detective wavelength 210 nm; methanol water of 25 ： 75 （V： V） has been taken as the mobile phase with the flow rate 0.5 mL/min. Under such experimental conditions, urotopine has a good linearity between 10-500 mg/L （r = 0.999 1） , the means of recovery rate is 96.45% and the relative standard deviations （n = 5） is 1.60%. This method is proved sensitive, accurate, reliable and applicable to determination of urotopine in yuba.
出处
《洛阳理工学院学报（自然科学版）》
2013年第3期1-4,共4页
Journal of Luoyang Institute of Science and Technology：Natural Science Edition
基金
广西科技攻关项目(桂科攻12118011-1)
关键词
腐竹
乌洛托品
高效液相色谱
yuba
urotopine
high performance liquid chromatography
