摘要
本文研究了压力对六齿金线鱼鱼糜凝胶的凝胶特性、溶解度、电泳和TCA-可溶性肽含量的影响,并与热处理的结果对比。研究表明,压力诱导凝胶的凝胶强度随压力增大而增大,600MPa处理的凝胶强度达到最大值为429.775 g·cm,比热处理凝胶高23.03%。与热处理样相比,压力诱导凝胶的硬度、咀嚼性较低,内聚性较高,弹性在200 MPa以上时较高,持水性较好,白度较低。压力诱导凝胶的溶解度高于热处理凝胶,SDS-PAGE显示其肌球蛋白重链较深,表明压力会使内源性转谷氨酰胺酶的活性降低,不利于形成ε-(γ-谷氨酰)赖氨酸键。压力诱导凝胶的TCA-可溶性肽含量低于热处理凝胶,表明压力可抑制引起鱼糜凝胶劣化的内源性水解蛋白酶的活性。结果表明,超高压处理有利于形成柔软、有弹性和凝胶强度高的凝胶;对内源性水解蛋白酶活性的抑制作用可能是超高压改善其凝胶特性的主要原因之一。
Effect of pressures on the textural properties, solubility, SDS-1oolyacrylamide gel electrophoresis （SDS-PAGE） and TCA-soluble peptides of gels from threadfin bream （Nemipterus bleekeri） surimi were investigated and compared with heat-induced surimi gels. According to the result, the increased gel strength of surimi gels was observed with the increasing of the pressure. Surimi gels induced by pressure 600 MPa possessed the highest gel-strength of 429.775 g.cm, being 23.03% higher than that of the heat-induced gel. Pressure-induced surimi gels had significant lower hardness and chewiness but higher cohesiveness than heat-induced gels. Higher springiness could be achieved when pressure was beyond 200 MPa. Pressure-induced surimi gels had higher water-holding ability but lower whiteness than heat-induced gels. Higher solubility and MHC band intensity of surimi gels treated with pressurization were observed than those of gels treated with heating, indicating that endogenous transglutaminase might be inactived under pressurization and thus result in the decrease of ε -（ γ -glutamyl） lysine linkage formation. The content of TCA-soluble peptide of pressure-induced gels were lower than that of heat-induced gels, suggesting the endogenous proteolysis protease in surimi which could result in gel-weakening might be inactived by high pressure. The above results demonstrated that tender surimi gels with higher springiness and gel strength could be induced by high pressure, which might be due to its inhibitory effect on the activity of the endogenous proteolysis proteinase in surimi.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第9期2058-2062,共5页
Modern Food Science and Technology
基金
国家自然科学基金资助项目(31101311)
关键词
超高压
六齿金线鱼
鱼糜
凝胶
ultra-high pressure
threadfin bream
surimi
gelatin
