摘要
目的建立兔邻近腹主动脉VX2肝癌模型,为高强度聚焦超声(HIFU)消融肝脏大血管旁肿瘤研究选择最佳建模时间点提供实验依据。方法采用手术直视下组织块包埋法对45只新西兰大白兔于肝门处接种VX2瘤块,接种后第14、21、28天行MRI,观察成瘤率、肿瘤体积和肿瘤-腹主动脉间距。结果接种后动物存活率为92.11%(41/45),成瘤率为95.12%(39/41)。MRI示接种后第14天T2WI呈高信号,增强后VIBE FS TlWI示肿瘤明显强化;第21天肿瘤T2WI呈高信号,肿瘤不均匀强化;第28天肿瘤边缘环形强化,中心无强化,肿瘤出现继发性坏死;肿瘤体积随时间逐渐增大,肿瘤-腹主动脉间距逐渐减小,差异均有统计学意义(P均<0.05)。结论直视下组织块包埋法建立兔邻近腹主动脉VX2肝癌模型操作简单、重复性好、成功率高,肿瘤接种后21天为研究HIFU消融肝脏大血管旁肿瘤作用的最佳时间点。
Objective To establish rabbit models of VX2 liver tumor adjacent to abdominal aorta, in order to offer experi- mental evidences for choosing the optimal time for HIFU ablation of tumor. Methods Pieces of VX2 tumor were implanted into the hepatic hilum in 45 rabbits with open surgery. All the rabbits underwent MRI on the 14th, 21st and 28th day after implantation, and the rate of tumor formation, the tumor volume and the distance between the tumor and abdominal aorta were observed. Results The survival rate and tumor formation rate was 92.11% （41/45） and 95.12% （39/41）, respec- tively. MRI showed that the tumor manifested as high signal on T2WI and obvious enhanced on VIBE FS T1W1 on the 14th and 21st day after implantation. Ring enhancement of tumor edge was detected, but no enhancement as found in tumor cen- ter on the 28th day after implantation, which suggesting secondary necrosis. The tumor volume increased and the distance between tumor and abdominal aorta decreased with time going （all P〈0.05）. Conclusion Establishing rabbit models of VX2 liver tumor adjacent to abdominal aorta by implanting tumor fragment with open surgery is feasible and reliable. The optimal time point for HIFU ablation research of liver tumor adjacent to abdominal aorta is the 21st day after implantation.
出处
《中国介入影像与治疗学》
CSCD
2013年第10期629-632,共4页
Chinese Journal of Interventional Imaging and Therapy
基金
重庆市科技计划项目(CSTC2011jjA0221)
关键词
肝脏肿瘤
磁共振成像
模型
动物
Liver neoplasms
Magnetic resonance imaging
Models, animal
