摘要
目的:观察全氟化碳对大鼠心肌缺血再灌注损伤的预防作用。方法:将45只雄性Wistar大鼠随机分为假手术组、缺血再灌注组与全氟化碳组。全氟化碳组在缺血前48h每天腹腔注射全氟化碳(30ml/kg)。结扎大鼠冠状动脉左前降支,使心肌缺血30min,再灌注120min,制成心肌缺血再灌注损伤模型。分别测定血清中乳酸脱氢酶(LDH)、超氧化物岐化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)水平,并行HE染色观察缺血心肌的病理变化。结果:与假手术组比较,缺血再灌注组LDH、MDA、MPO值均增高,而SOD活性降低(P<0.05);与缺血再灌注组比较,全氟化碳组LDH、MDA、MPO值均降低,而SOD活性增高(P<0.05),且心肌形态学改变更小。结论:全氟化碳对心肌缺血再灌注损伤有预防作用,可能与抗氧化和抗炎症反应有关。
Objective：To observe the protective effects of perfluorocarbons on myocardial ischemial and reper- fusion injury in rats. Method： The 45 male wistar rats were divided randomly into sham operation group, myocardi- al ischemia and reperfused injury （IRI） group and perfluorocarbon （PFC） group. Perfluorocarbons （30 ml/kg） were intraperitoneally injected 48 hours before ischemia everyday. The left anterior descending branch of coronary artery was ligated for 30 minutes and reperfused for 2 hours to build the myocardial ischemial and reperfusion inju- ry model. The changes of lactate dehydrogenase （LDH）, superoxide dismutase （SOD）, malondialdehyde （MDA）, myeloperoxidase （MPO） levels were determined, and the pathological changes of myocardium were observed by HE stain. Result： Compared with sham operation group, LDH, MDA and MPO increased and SOD decreased in IRI group （P^0.05）. Compared with IRI group, LDH, MDA and MPO decreased and SOD inecreased in PFC group （P^0.05）, and PFC inhibited the histomorphological changes of ischemic myocardium. Conclusion：perfluorocarbon relieves the ischemia and reperfusion injury and the cardioprotection may be due to antioxidant effects and antiinflammatory reaction.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2013年第9期705-707,共3页
Journal of Clinical Cardiology
关键词
全氟化碳
心肌缺血再灌注
抗氧化
抗炎症反应
perfluorocarbon
ischemial and reperfusion injury
antioxidant
antiinflammatory reaction
