摘要
目的探讨全血γ干扰素诱导蛋白-10(IP-10)对活动性结核病的辅助诊断价值。方法检测66例活动性肺结核病患者、40例非结核呼吸疾病患者及40例健康对照者血浆中非特异性IP-10的水平;分别应用纯化的结核分枝杆菌特异性蛋白早期分泌性靶抗原-6(EAST-6)和培养液蛋白10(CFP10)体外刺激患者全血,检测活动性结核病组、非结核呼吸疾病组和健康对照组人群全血中IP-10的释放水平;绘制受试者工作特征曲线,比较非特异性IP-10和特异性IP-10对活动性肺结核的诊断效能。结果活动性结核病组患者血浆中非特异性IP-10的水平为(139.6±124.2)pg/mL,明显高于健康对照组[(33.5±17.7)pg/mL,P<0.05];而与非结核呼吸疾病组[(88.1±73.3)pg/mL]无明显差别(P>0.05)。经ESAT-6及CFP10诱导后,活动性结核病组患者全血IP-10释放水平为(146.0±167.1)pg/mL,显著高于非结核呼吸疾病组[(26.6±9.7)pg/mL,P<0.01]与健康对照组[(24.2±9.7)pg/mL,P<0.01]。经过ROC分析,结核特异性的IP-10诊断结核病的临界值为41.2 pg/mL,敏感度为68.2%,特异度为93.7%。特异性IP-10 ROC曲线下面积为0.905,高于非特异性IP-10(0.747,P<0.01)。结论 IP-10可作为活动性结核病的辅助诊断方法。采用结核特异性IP-10较血浆非特异性IP-10能够提高活动性肺结核的诊断效能。
Object ve To determine the clinical value of interferon-inducible protein 10 （IP-10） in auxiliary diagnosis of active tuberculosis. Methods The level of non-specific IP-10 was evaluated in the plasma of 66 active tuberculosis patients, 40 non-pulmonary tuberculous patients and 40 healthy controls, and the level of specific IP-10 was detected in the whole blood after stimulated by purified mycobacterium tuberculosis-specific antigen ESAT6 and culture filtrate protein 10 （CFP10）. Then the receiver operating characteristic （ROC） curve was drawn to determine the diagnostic value of non-specific IP-10 and specific IP-10 to detect active tuberculosis. Results The non-specific IP-IO level of the active tuberculosis group （139.6 ± 124.2 pg/mL） was obviously higher than that of the healthy control group （33.5 _± 17.7 pg/mL, P 〈 0.05 ）, but had no difference with that of the non-tuberculous pulmonary disease group （ 88.1 ± 73.3 pg/mL, P 〉0. 05 ）. After stimulated by the ESAT6 and CFP10, the specific IP-10 level of active tuberculosis group （ 146.0 ± 167.1 pg/mL） was signifieantly higher than that of non-tubereulous pulmonary disease group （26. 6 ± 9.7 pg/mL, P 〈0.01 ） and that of the healthy control group （24.2 ± 9.7 pg/mL, P 〈 0.01 ）. After ROC analysis, the threshold of the speeifie IP-10 for diagnosis of tuberculosis was 41.1 pg/mL and its sensitivity and specificity were 70.8% and 91.8% respectively. The area under curve （AUC） of the speeifie IP-10 was 0. 905, which was obviously higher than that of non-speeific IP-10 （0. 747, P 〈 0.01 ）. Conclusion The gamma interferon-indueible protein 10 release assay can be used as auxiliary diagnostic method for aetive tuber- culosis. The diagnostie value of the speeific IP-10 is better than that of non-specific IP-10.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2013年第19期2092-2094,共3页
Journal of Third Military Medical University
基金
国家自然科学基金面上项目(30771037)~~
关键词
γ干扰素诱导蛋白-10
肺结核
诊断
gamma interferon-inducible protein 10
pulmonary tuberculosis
diagnosis
