摘要
目的：建立一种高效液相色谱法测定铁皮枫斗颗粒中柚皮素含量的方法。方法：色谱柱UltimateXB—C18（250mm×4．6mm，5μm）；流动相为0．2％H3PO4的水溶液与甲醇采用梯度洗脱；流速为1．0mL·min^-1；检测波长为280nm；柱温30℃。结果：柚皮素的回归方程为Y=3227．6X-1．7272（r=1．000），线性范围：0．005～0．075μg，加样回收率：100．82％（RSD=1．06％，n=6）。结论：该法准确，阴性制剂无干扰，精密度高，重复性好。可用于铁皮枫斗颗粒中柚皮素的含量测定，并为其它铁皮枫斗相关制剂质量检测提供参考。
Objective：To establish a new HPLC method to determine naringenin in Dendrobium Officinale Granules. Methods：The sample was detected on a ultimate C18 column（250 mm × 4.6 mm,5 μm）with the mobile phase consisting with 0.2% phosphoric acid and methanol by gradient elution at 30 ℃. The flow rate was 1.0 mL · min^-1 ,and wavelength was 280 nm. Results ： The standard curve for naringenin was linear over the range from 0. 005 μg to 0. 075 txg （ r = 1. 000） ,typical equation： Y = 3227.6X - 1. 7272, the average recovery was 100.82% （ RSD = 1.06 % , n = 6）. Conclu- sion：The method shows good accuracy, precision and repeatability. It can be used by quality control of D. Officinale Gran- ules and can be taken as a reference for other connected preparations.
出处
《中华中医药学刊》
CAS
2013年第9期1855-1857,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家“十二五”科技支撑计划资助项目(2011BAI04B06)
浙江省卫生高层次创新人才培养工程项目(浙卫发[2010]190号)
浙江省教育厅资助项目(Y201122310)
浙江省重大科技专项项目(2006C13014)
关键词
铁皮枫斗颗粒
铁皮石斛
柚皮素
含量测定
Dendrobium Officinale Granule
Dendrobium officinale Kimura et Migo
naringenin
content determination
