摘要
尝试使用免疫学的方法来防治对虾弧菌病。将病原副溶血弧菌制成福尔马林灭活疫苗，在实验条件下分别以浸泡、口服和注射等方式接种于斑节对虾和日本对虾，结果发现，在一定的范围内，对虾的免疫保护率随疫苗浓度的增加而升高。在注射接种的方式中，斑节对虾在 １０９、１０８、１０７ｃｅｌｌｓ·ｍＬ－１的疫苗剂量下的保护力分别为４０％、５０％和４０％，浸泡组（疫苗浓度分别为１０８ｃｅｌｌｓ·ｍＬ－１、１０７ｃｅｌｌｓ·ｍＬ－１和１０６ｃｅｌｌｓ·ｍＬ－１）的免疫保护率分别为３０％、３０％和２０％，投喂组的免疫保护率为１１．１％。日本对虾注射免疫组（疫苗剂量为１０８ｃｅｌｌｓ·ｍＬ－１、１０７ｃｅｌｌｓ· ｍＬ－１和 １０６ｃｅｌｌｓ· ｍＬ－１）的免疫保护率分别为 ６０％、４０％和 ３０％，浸泡组（疫苗浓度分别为１０８ｃｅｌｌｓ·ｍＬ－１、１０ｃｅｌｌｓ·ｍＬ－１和１０６ｃｅｌｌｓ·ｍＬ－１）的分别为２２．２％、２２．２％和１１．１％，投喂组的免疫保护率为０。表明疫苗能提高对虾的成活率和抗感染能力，初步证实了对虾弧菌灭活疫苗的有效性，为日后大规模的生产实践打下了基础。
For Preventing penaeid shrimps vibrosis, the immunological methods are studied. Vaccines are made of killed bacteria by formalin. Shrimps inoculated by immersion, intramuscular injection and oral administration are more resistant to vibrosis than those not inoculated. Within a certain extent, the resistance to vibrosis of penaeid shrimps inoculated improves with the increasing of vaccine concentration. For Penaeus monodon by intramuscular injection, the RPS (Relative Percent of Survival) is 40% .50% and 40% respectively in vaccine concentration of 109.108. 107cells. mL-l, the RPS is 30% .30% and 20% respectively in vaccine concentration of 108 cells' mL-1 .107cells.mL- 1 and 106cells.mL-1 by immersion, the RPS is 11. l% by oral administration. For Penaeus japonicus by intramuscular injection, the RPS is 60%.40% and 30% respectively in vaccine concentration of 108cells.mL-l' 107cells. 'mL-l and 106cells. mL-l, the RPS is 22. 2% .22. 2% 和 11. l% respectively in vaccine concentration of 108cells.mL-1 .107cells'mL- 1 and 106cells.mL- 1 by immersion, the RPS is 0 by oral administration. The result suggested that the vaccine can improve penaeid shrimps survival rate and anti-infected ability.
出处
《水产学报》
CAS
CSCD
北大核心
2000年第6期564-569,共6页
Journal of Fisheries of China
基金
中国科学院"九五"重大科研资助项目!(K2951-B-111)
中国科学院生物科学与技术特别支持费项目!(Y982201)
