摘要
采用NaNO2-A l(N O3)3-N aOH显色法测定药桑总黄酮含量,紫外可见分光光度法测定药桑中总黄酮对DPPH·、ABTS·+的清除率,SPSS统计软件分析黄酮与抗氧化活性之间的相关性,探讨药桑中总黄酮的含量与抗氧化活性的相关性。结果显示药桑不同溶剂萃取部位中总黄酮含量各不相同,其中乙酸乙酯相总黄酮含量最高,水相中总黄酮含量最低。通过SP PS17.0分析,发现总黄酮的含量与抗氧化活性之间的关系是:与D PPH·清除率呈正相关关系,为显著相关关系,与A BT S·+清除率呈正相关关系,但相关性不显著。本研究初步确定了药桑中总黄酮的含量与其抗氧化化活性的关系。
In order to clarify the correlation between the content and antioxidant activity of total flavonoids in Morus nigra Linn. ,the content of flavonoids was determined by the colorimetric method of NaNO2-A1 （NO3）3-NaOH. The antioxidant activity ＇was analysed by UV. And the correlation between them was analysed by SPSS. The result shows that the content of the total flavonoids from different extraction parts of Morus nigra l.inn, are not identical. The total flavonoids content of ethyl acetate phase is the highest, and aqucoue phase is the lowest. The correlation between the total flavonoids; content and the antioxidant activity shows that the total flavonoids; content with the removal rate of DPPH ; and ABTS· ＋ are positively correlated,and as the significant correlation with DPPH ; ,but the correlation is not significant for ABTS ·＋
出处
《光谱实验室》
CAS
2013年第5期2644-2648,共5页
Chinese Journal of Spectroscopy Laboratory
基金
国家大学生创新创业训练计划项目(201210757054)
关键词
药桑
总黄酮
抗氧化活性
相关性
Morus nigra l.inn.
Tolal Flavonoids
Antioxidant Activity
Correlation
